Mouse anti-Human DOC2A Monoclonal Antibody | anti-DOC2A antibody

DOC2A (Double C2-like Domain-containing Protein alpha, Doc2, Doc2-alpha) (FITC)

Cat. Num. AAA6146894

• Gene Names: DOC2A; Doc2
• Reactivity: Human
• Applications: ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DOC2A; Monoclonal Antibody; DOC2A (Double C2-like Domain-containing Protein alpha; Doc2; Doc2-alpha) (FITC); anti-DOC2A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 3C11
• Specificity: Recognizes human DOC2A.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).

• Applicable Applications for anti-DOC2A antibody: ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-401 from DOC2A (AAH63436) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MRGRRGDRMTINIQEHMAINVCPGPIRPIRQISDYFPRGPGPEGGGGSGGEAPAHLVPLALAPPAALLGATTPEDGAEVDSYDSDDATALGTLEFDLLYDRASCTLHCSILRAKGLKPMDFNGLADPYVKLHLLPGACKANKLKTKTQRNTLNPVWNEDLTYSGITDDDITHKVLRIAVCDEDKLSHNEFIGEIRVPLRRLKPSQKKHFNICLERQVPLASPSSMSAALRGISCYLKELEQAEQGQGLLEERGRI
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged DOC2A is 1ng/ml as a capture antibody.)

Related Product Information for anti-DOC2A antibody

Doc2-alpha is mainly expressed in brain and is suggested to be involved in Ca(2+)-dependent neurotransmitter release. It may also be involved in dynein-dependent intracellular vesicle transport. The cDNA predicts a 400-amino acid protein with relative mass of 44,071 Da and 2 repeated C2-like domains in the C-terminal region. The C2 domain 1 is involved in binding calcium and phospholipids. It is predominantly expressed in the brain but also expressed in testis.

Product Categories/Family for anti-DOC2A antibody

Antibodies; Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 39645113
• NCBI GeneID: 8448
• Molecular Weight: 18,384 Da
• NCBI Official Full Name: Homo sapiens double C2-like domains, alpha, mRNA
• NCBI Official Synonym Full Names: double C2 domain alpha
• NCBI Official Symbol: DOC2A
• NCBI Official Synonym Symbols: Doc2
• NCBI Protein Information: double C2-like domain-containing protein alpha
• Protein Family: Double C2-like domain-containing protein

NCBI Description

There are at least two protein isoforms of the Double C2 protein, namely alpha (DOC2A) and beta (DOC2B), which contain two C2-like domains. DOC2A and DOC2B are encoded by different genes; these genes are at times confused with the unrelated DAB2 gene which was initially named DOC-2. DOC2A is mainly expressed in brain and is suggested to be involved in Ca(2+)-dependent neurotransmitter release. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]