Rabbit anti-Human E2F-6 Polyclonal Antibody | anti-E2F-6 antibody

E2F-6 (E170) polyclonal antibody

Cat. Num. AAA3014581

• Gene Names: E2F6; E2F-6
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. The purity is >95% (by SDS-PAGE).
• Synonyms: E2F-6; Polyclonal Antibody; E2F-6 (E170) polyclonal antibody; Transcription factor E2F6; E2F6; anti-E2F-6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: E2F6 (E170) polyclonal antibody detects endogenous levels of E2F6 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. The purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Applicable Applications for anti-E2F-6 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 141-190 of Human E2F-6.
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of E2F6 pAb at 1:500 dilutionLane1:A2780 whole cell lysate(40ug)Lane2:HEK293T whole cell lysate(40ug)Lane3:HCT116 whole cell lysate(40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of E2F-6 (E170) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.)

Related Product Information for anti-E2F-6 antibody

The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcriptional regulators including E2F-2, E2F-3, E2F-4, E2F-5 and E2F-6, each of which forms heterodimers with a second protein, DP-1, forming an "active" E2F transcriptional regulatory complex.

NCBI and Uniprot Product Information

• NCBI GI #: 109637795
• NCBI GeneID: 1876
• NCBI Accession #: NP_937987.2
• NCBI GenBank Nucleotide #: NM_198256.3
• UniProt Accession #: O75461; O60544; Q53QY9; Q7Z2H6; A8K2Z8
• Molecular Weight: 31,844 Da
• NCBI Official Full Name: transcription factor E2F6 isoform 1
• NCBI Official Synonym Full Names: E2F transcription factor 6
• NCBI Official Symbol: E2F6
• NCBI Official Synonym Symbols: E2F-6
• NCBI Protein Information: transcription factor E2F6
• UniProt Protein Name: Transcription factor E2F6
• UniProt Gene Name: E2F6
• UniProt Synonym Gene Names: E2F-6
• UniProt Entry Name: E2F6_HUMAN

NCBI Description

This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Uniprot Description

E2F6: Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase. Belongs to the E2F/DP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 2p25.1

Cellular Component: transcription factor complex; nucleus

Molecular Function: DNA binding; transcription corepressor activity; transcription factor activity

Biological Process: G1/S-specific transcription in mitotic cell cycle; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter