Rabbit HSP60 Polyclonal Antibody | anti-HSPD1 antibody

Anti-HSP60 antibody

Cat. Num. AAA175435

• Gene Names: Hspd1; Hsp60; Hspd1-30p
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Immunogen affinity purified.
• Synonyms: HSP60; Polyclonal Antibody; Anti-HSP60 antibody; 60 kDa heat shock protein; mitochondrial; heat shock 60kDa protein 1(chaperonin); 60 kDa chaperonin antibody; 60 kDa heat shock protein mitochondrial antibody; mitochondrial antibody; CH60_HUMAN antibody; Chaperonin 60 antibody; Chaperonin antibody; Chaperonin; 60-KD antibody; CPN 60 antibody; CPN60 antibody; fa04a05 antibody; GROEL antibody; GroEL Homolog antibody; Heat shock 60kD protein 1 chaperonin antibody; Heat shock 60kDa protein 1 antibody; Heat shock protein 1(chaperonin) antibody; Heat Shock Protein 60 antibody; Heat shock protein 65 antibody; HLD4 antibody; Hsp 60 antibody; HSP 65 antibody; HSP-60 antibody; HSP60 antibody; HSP65 antibody; HSPD 1 antibody; HSPD1 antibody; HuCHA60 antibody; Mitochondrial matrix protein P1 antibody; P60 lymphocyte protein antibody; Short heat shock protein 60 Hsp60s1 antibody; Spastic paraplegia 13 antibody; SPG 13 antibody; SPG13 antibody; anti-HSPD1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 573

• Applicable Applications for anti-HSPD1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of rat HSP60 (503-516aa YDAMLGDFVNMVEK), different from the related human sequence by one amino acid.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-HSP60 antibody, MBS175435, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Liver Tissue LysateLane 3: CEM Cell LysateLane 4: HELA Cell LysateLane 5: SMMC Cell LysateLane 6: COLO320 Cell Lysate )

Immunohistochemistry (IHC)

(Anti-HSP60 antibody, MBS175435, IHC(P)IHC(P): Rat Intestine Tissue )

Immunocytochemistry (ICC)

(Anti-HSP60 antibody, MBS175435, ICCICC: SMMC Cell )

Related Product Information for anti-HSPD1 antibody

Description: Rabbit IgG polyclonal antibody for 60 kDa heat shock protein, mitochondrial(HSPD1) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.
Background: HSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling.

NCBI and Uniprot Product Information

• NCBI GI #: 51702230
• NCBI GeneID: 63868
• UniProt Accession #: P63039; P19226; P19227; P97602
• Molecular Weight: 60,955 Da
• NCBI Official Full Name: 60 kDa heat shock protein, mitochondrial
• NCBI Official Synonym Full Names: heat shock protein 1 (chaperonin)
• NCBI Official Symbol: Hspd1
• NCBI Official Synonym Symbols: Hsp60; Hspd1-30p
• NCBI Protein Information: 60 kDa heat shock protein, mitochondrial; CPN60; HSP-60; HSP-65; chaperonin 60; 60 kDa chaperonin; heat shock 60kDa protein 1; heat shock protein 60 (liver); mitochondrial matrix protein P1; heat shock 60kD protein 1 (chaperonin)
• UniProt Protein Name: 60 kDa heat shock protein, mitochondrial
• Protein Family: 60 kDa heat shock protein
• UniProt Gene Name: Hspd1
• UniProt Synonym Gene Names: Hsp60; CPN60; HSP-60; Hsp60
• UniProt Entry Name: CH60_RAT

NCBI Description

a stress-inducible molecular chaperone which participates in protein folding [RGD, Feb 2006]

Uniprot Description

HSP60: Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. Interacts with HRAS. Interacts with HBV protein X and HTLV-1 protein p40tax. Belongs to the chaperonin (HSP60) family.

Protein type: Chaperone; Mitochondrial

Cellular Component: Golgi apparatus; extracellular space; protein complex; cell surface; rough endoplasmic reticulum; intracellular membrane-bound organelle; mitochondrion; early endosome; coated pit; cytosol; lipid raft; secretory granule; mitochondrial crista; membrane; mitochondrial matrix; mitochondrial inner membrane; cytoplasm; plasma membrane; lipopolysaccharide receptor complex; cyclin-dependent protein kinase activating kinase holoenzyme complex; myelin sheath

Molecular Function: insulin binding; protein binding; protease binding; protein heterodimerization activity; lipopolysaccharide binding; p53 binding; chaperone binding; ubiquitin protein ligase binding; double-stranded RNA binding; protein complex binding; misfolded protein binding; ATP binding

Biological Process: B cell proliferation; T cell activation; positive regulation of apoptosis; positive regulation of interleukin-12 production; isotype switching to IgG isotypes; response to lipopolysaccharide; response to organic cyclic substance; positive regulation of interleukin-10 production; response to organic substance; protein refolding; negative regulation of neuron apoptosis; positive regulation of T cell mediated immune response to tumor cell; positive regulation of interferon-alpha production; positive regulation of macrophage activation; caspase activation; response to drug; B cell activation; chaperone cofactor-dependent protein folding; protein stabilization; positive regulation of interleukin-6 production; B cell cytokine production; detection of misfolded protein; response to cocaine; response to unfolded protein; response to ATP; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interferon-gamma production; 'de novo' protein folding; response to hydrogen peroxide; response to estrogen stimulus; response to heat; response to hypoxia; positive regulation of T cell activation; negative regulation of apoptosis; positive regulation of inflammatory response