Testing Data
(Figure 1: Immunohistochemistry on frozen section of human colon
)
Mouse CytokeRatin 8 / KeRatin K8 Monoclonal Antibody | anti-KRT8 antibody
Mouse anti CytokeRatin 8 / KeRatin K8
Gene Names
KRT8; K8; KO; CK8; CYK8; K2C8; CARD2
Reactivity
Human, Rabbit, Rat
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Immunohistochemistry, Western Blot
Synonyms
CytokeRatin 8 / KeRatin K8; Monoclonal Antibody; Mouse anti CytokeRatin 8 / KeRatin K8; anti-KRT8 antibody
Host
Mouse
Reactivity
Human, Rabbit, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M20
Specificity
M20 reacts with glandular epithelial cells (endocrine and exocrine) as well as mesothelial cells and epithelia of the digestive, respiRatory and urogenital tract. Also most adenocarcinomas derived from these cells are positive. The M20 epitope has been mapped to amino acids 353-367 on the alpha helical rod domain of cytokeRatin 8 (see reference 10).
Form/Format
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-KRT8 antibody
Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)
Application Notes
M20 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues and flow cytometry. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations.
Source Note
M20 is a Mouse monoclonal IgG1 antibody derived by fusion of murine myeloma cells with spleen cells from a Mouse immunized with keRatin isolated from the Human breast carcinoma cell line MCF-7.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Testing Data
(Figure 1: Immunohistochemistry on frozen section of human colon )
Testing Data
(Figure 1: Immunohistochemistry on frozen section of human colon
)
Testing Data
(Figure 2: Immunohistochemistry on paraffin section of human colon )
Testing Data
(Figure 2: Immunohistochemistry on paraffin section of human colon
)
Related Product Information for anti-KRT8 antibody
CytokeRatins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual cytokeRatin polypeptides are numbered 1 to 20. The various epithelia in the Human body usually express cytokeRatins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. CytokeRatin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeRatin antibodies are not only of assistance in the differential identification of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Product Categories/Family for anti-KRT8 antibody
References
Van Muijen, G. N., Ruiter, D. J., and Warnaar, S. O. (1987). Coexpression of intermediate filament polypeptides in Human fetal and adult tissues. Lab Invest 57, 359-69. Van Muijen, G. N., Warnaar, S. O., and Ponec, M. (1987). Differentiation-related changes of cytokeRatin expression in cultured keRatinocytes and in fetal, newborn, and adult epidermis. Exp Cell Res 171, 331-45. Schaafsma, H. E., Ramaekers, F. C., van Muijen, G. N., Ooms, E. C., and Ruiter, D. J. (1989). Distribution of cytokeRatin polypeptides in epithelia of the adult Human urinary tract. Histochemistry 91, 151-9. Smedts, F., Ramaekers, F., Robben, H., Pruszczynski, M., van Muijen, G., Lane, B., Leigh, I., and Vooijs, P. (1990). Changing patterns of keRatin expression during progression of cervical intraepithelial neoplasia. Am J Pathol 136, 657-68. Ramaekers, F., van Niekerk, C., Poels, L., Schaafsma, E., Huijsmans, A., Robben, H., Schaart, G., and Vooijs, P. (1990). Use of monoclonal antibodies to keRatin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol 136, 641-55. Schaafsma, H. E., Ramaekers, F. C., van Muijen, G. N., Lane, E. B., Leigh, I. M., Robben, H., Huijsmans, A., Ooms, E. C., and Ruiter, D. J. (1990). Distribution of cytokeRatin polypeptides in Human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression. Am J Pathol 136, 329-43. Ivanyi, D., Groeneveld, E., Van Doornewaard, G., Mooi, W. J., and Hageman, P. C. (1990). KeRatin subtypes in carcinomas of the uterine cervix: impliCations for histogenesis and differential diagnosis. Cancer Res 50, 5143-52. Wetzels, R. H., Kuijpers, H. J., Lane, E. B., Leigh, I. M., Troyanovsky, S. M., Holland, R., van Haelst, U. J., and Ramaekers, F. C. (1991). Basal cell-specific and hyperprolifeRation-related keRatins in Human breast cancer. Am J Pathol 138, 751-63. Ku, N. O., Gish, R., Wright, T. L., and Omary, M. B. (2001). KeRatin 8 mutations in patients with cryptogenic liver disease. N Engl J Med 344, 1580-7. Waseem, A., Karsten, U., Leigh, I.M., Purkis, P., Waseem, H. and Lane, B. (2004). Conformational changes in the rod domain of Human keRatin 8 following heterotypic association with keRatin 18 and its impliCation for filament stability. Biochemistry 43, 1283-95.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
53,704 Da
NCBI Official Full Name
cytokeratin 8
NCBI Official Synonym Full Names
keratin 8
NCBI Official Symbol
KRT8
NCBI Official Synonym Symbols
K8; KO; CK8; CYK8; K2C8; CARD2
NCBI Protein Information
keratin, type II cytoskeletal 8; CK-8; keratin-8; cytokeratin 8; cytokeratin-8; type-II keratin Kb8
UniProt Protein Name
Keratin, type II cytoskeletal 8
Protein Family
UniProt Gene Name
KRT8
UniProt Synonym Gene Names
CYK8
UniProt Entry Name
K2C8_HUMAN
NCBI Description
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq]
Uniprot Description
K8: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Phsophorylated by p38 kinase, regulating cellular keratin filament reorganization. Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Mutation of this protein is a risk factor for cryptogenic liver failure.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 12q13
Cellular Component: nucleoplasm; dystrophin-associated glycoprotein complex; intermediate filament cytoskeleton; costamere; nuclear matrix; cytoplasm; keratin filament; intermediate filament; intercellular junction; nucleus; Z disc; sarcolemma
Molecular Function: protein binding; protein complex binding; structural molecule activity
Biological Process: tumor necrosis factor-mediated signaling pathway; viral reproduction; sarcomere organization; response to other organism; response to hydrostatic pressure
Disease: Cirrhosis, Familial
Research Articles on KRT8
Similar Products
Product Notes
The KRT8 krt8 (Catalog #AAA570161) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti CytokeRatin 8 / KeRatin K8 reacts with Human, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CytokeRatin 8 / KeRatin K8 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB). M20 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues and flow cytometry. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations. Researchers should empirically determine the suitability of the KRT8 krt8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CytokeRatin 8 / KeRatin K8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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