Mouse anti-Human, Mouse CSNK2A2 Monoclonal Antibody | anti-CSNK2A2 antibody

CSNK2A2

Cat. Num. AAA6013075

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay
• Purity: Ascites
• Synonyms: CSNK2A2; Monoclonal Antibody; Anti -CSNK2A2; anti-CSNK2A2 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 13B201
• Specificity: Human, Mouse
• Purity/Purification: Ascites
• Form/Format: Ascites fluid, 0.03% sodium azide.

• Applicable Applications for anti-CSNK2A2 antibody: ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Western Blot: 1:500-1:2000, Flow Cytometry: 1:200-1:400, ELISA: 1:10000
• Immunogen: Purified recombinant fragment of human CSNK2A2 (AA: 194-350) expressed in E. coli.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-CSNK2A2 antibody

Casein kinase II (CK2) is a constitutively active, ubiquitously expressed serine:threonine protein kinasethat is thought to have a regulatory function in cell proliferation, cell differentiation and apoptosis. CK2functions as a tetrameric complex consisting of two regulatory beta-subunits and two catalytic units (alphaand alpha') in a homomeric or heteromeric conformation. Whilst the alpha- and alpha'-subunits arecatalytically identical, proteins that regulate CK2, such as cdc2 and Hsp90, preferentially bind to thealpha and not the alpha'-subunit. CK2 can phosphorylate a number of key intracellular signaling proteinsimplicated in tumor suppression (p53 and PTEN) and tumorigenesis (myc, jun, NF-kappaB). CK2 is also thoughtto influence Wnt signaling via beta-catenin phosphorylation and the PI 3-K signaling pathway via thephosphorylation of Akt.

Product Categories/Family for anti-CSNK2A2 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 27807145
• NCBI GeneID: 282420
• NCBI Accession #: NP_777061.1
• NCBI GenBank Nucleotide #: NM_174636.2
• UniProt Accession #: P20427
• Molecular Weight: 41,270 Da
• NCBI Official Full Name: casein kinase II subunit alpha'
• NCBI Official Synonym Full Names: casein kinase 2, alpha prime polypeptide<
• NCBI Official Symbol: CSNK2A2
• NCBI Protein Information: casein kinase II subunit alpha'; CK II alpha'
• UniProt Protein Name: Casein kinase II subunit alpha'
• Protein Family: Casein kinase
• UniProt Gene Name: CSNK2A2
• UniProt Synonym Gene Names: CK II alpha'
• UniProt Entry Name: CSK22_BOVIN

Uniprot Description

Function: Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins

By similarity.

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation: Constitutively active protein kinase whose activity is not directly affected by phosphorylation. Seems to be regulated by level of expression and localization

By similarity.

Subunit structure: Heterotetramer composed of two catalytic subunits (alpha chain and/or alpha' chain) and two regulatory subunits (beta chains). The tetramer can exist as a combination of 2 alpha/2 beta, 2 alpha'/2 beta or 1 alpha/1 alpha'/2 beta subunits. Also part of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, which forms following UV irradiation. Interacts with RNPS1

By similarity.

Miscellaneous: Can use both ATP and GTP as phosphoryl donors. Phosphorylation by casein kinase 2 has been estimated to represent up to one quarter of the eukaryotic phosphoproteome.

Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.Contains 1 protein kinase domain.