CSNK2A1 / CK2A1 recombinant protein

Recombinant Mouse CSNK2A1 / CK2A1 Protein (His & GST tag)

Cat. Num. AAA2546601

• Gene Names: Csnk2a1; Csnk2a1-rs4
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: CSNK2A1 / CK2A1; Recombinant Mouse CSNK2A1 / CK2A1 Protein (His & GST tag); Csnk2a1; Ckiia; CSNK2A1 / CK2A1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly
• Sequence Length: 391

• Application Notes: The secreted recombinant mouse CSNK2A1/GST chimera consists of 628 amino acids and has a calculated molecular mass of 72.9 kDa. The recombinant protein migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CSNK2A1 / CK2A1 recombinant protein

Background: Casein kinase II subunit alpha, also known as CK II alpha, CSNK2A1 and CK2A1, is a member of the protein kinase superfamily, Ser / Thr protein kinase family and CK2 subfamily. Casein kinase II (CSNK2A1) is a serine / threonine protein kinase that phosphorylates acidic proteins such as casein. This kinase is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. Casein kinase II (CSNK2A1) is a constitutively active, ubiquitously expressed serine / threonine protein kinase that is thought to have a regulatory function in cell proliferation, cell differentiation and apoptosis. CSNK2A1 functions as a tetrameric complex consisting of two regulatory beta-subunits and two catalytic units (alpha and alpha') in a homomeric or heteromeric conformation. Whilst the alpha- and alpha'-subunits are catalytically identical, proteins that regulate CSNK2A1, such as cdc2 and Hsp90, preferentially bind to the alpha and not the alpha'-subunit. CSNK2A1 can phosphorylate a number of key intracellular signaling proteins implicated in tumor suppression (p53 and PTEN) and tumorigenesis (myc, jun, NF-kappaB). CSNK2A1 is also thought to influence Wnt signaling via beta-catenin phosphorylation and the PI 3-K signaling pathway via th phosphorylation of Akt.

Description: A DNA sequence encoding the mouse CSNK2A1 (Q60737) (Met1-Gln391) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 31542427
• NCBI GeneID: 12995
• NCBI Accession #: NP_031814.2
• NCBI GenBank Nucleotide #: NM_007788.3
• UniProt Accession #: Q60737; Q8R0X4
• Molecular Weight: 45,134 Da
• NCBI Official Full Name: casein kinase II subunit alpha
• NCBI Official Synonym Full Names: casein kinase 2, alpha 1 polypeptide
• NCBI Official Symbol: Csnk2a1
• NCBI Official Synonym Symbols: Csnk2a1-rs4
• NCBI Protein Information: casein kinase II subunit alpha
• UniProt Protein Name: Casein kinase II subunit alpha
• UniProt Gene Name: Csnk2a1
• UniProt Synonym Gene Names: Ckiia; CK II alpha

NCBI Description

Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythms. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. [provided by RefSeq, Feb 2014]

Uniprot Description

CK2A1: an ubiquitous protein kinase of the CK2 family. Exists as a tetramer composed of two catalytic subunits, alpha and alpha-prime, and two regulatory beta subunits. The beta subunits undergo autophosphorylation. The isoforms are rarely specified in publications. Two splice variant isoforms have been found. Participates in Wnt signaling. Phosphorylates E2 ubiquitin conjugating enzyme UBC3B inducing its interaction with beta-TRCp and enhancing beta-catenin degradation. May control IkB-alpha and p27Kip1 degradation. component of a CK2-SPT16-SSRP1 complex composed of SSRP1, PT16 CK2-A1, CK2-A2 and CK2-B the complex associating following UV irradiation. Interacts with RNPS1. Mouse transgene causes mammary gland hyperplasia and lymphoma, and activation by bovine parasites leads to fatal lymphoproliferation. Expression and activity are elevated in lung tumors and breast tumors. Antisense drives apoptosis of tumor cell lines and xenografts. Involved in DNA break repair by phosphorylation of scaffold protein XRCC1, phosphorylation of BRCA1, and phosphorylation of p53 in response to UV irradiation. Drosophila CK2 (Timekeeper) is involved in circadian regulation. Phosphorylates and binds to a major component of the inclusion bodies seen in Parkinson?s patients. Inhibitors: 4,5,6,7-tetrabromobenzotriazole (TBB).

Protein type: CK2 family; EC 2.7.11.1; Kinase, protein; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 2|2 G3

Cellular Component: nucleus; NuRD complex; PcG protein complex; protein kinase CK2 complex; Sin3 complex

Molecular Function: beta-catenin binding; kinase activity; protein binding; protein N-terminus binding; protein phosphatase regulator activity; protein serine/threonine kinase activity

Biological Process: negative regulation of caspase activity; positive regulation of cell growth; positive regulation of cell proliferation; positive regulation of protein catabolic process; positive regulation of Wnt receptor signaling pathway; protein amino acid autophosphorylation; protein amino acid phosphorylation