Rabbit anti-Human, Mouse Csnk2a2 Polyclonal Antibody | anti-Csnk2a2 antibody

Csnk2a2, NT (Csnk2a2, Casein kinase II subunit alpha') (PE)

Cat. Num. AAA6289406

• Gene Names: Csnk2a2; CK2; C77789; 1110035J23Rik
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Csnk2a2; Polyclonal Antibody; NT (Csnk2a2; Casein kinase II subunit alpha') (PE); Casein kinase II subunit alpha'; anti-Csnk2a2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-Csnk2a2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Csnk2a2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3-32 amino acids from the N-terminal region of Mouse Csnk2a2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Csnk2a2 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the Csnk2a2 antibody detected the Csnk2a2 protein (arrow).)

Western Blot (WB)

(Mouse Csnk2a2 Antibody (N-term) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the Csnk2a2 antibody detected the Csnk2a2 protein (arrow).)

Related Product Information for anti-Csnk2a2 antibody

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-389' of p53/TP53 following UV irradiation (By similarity).

Product Categories/Family for anti-Csnk2a2 antibody

Antibodies; Protein Kinases

References

Pastori, V., et al. Biochim. Biophys. Acta 1802 (7-8), 583-592 (2010): Cobb, L.J., et al. Mol. Endocrinol. 23(10):1624-1633(2009). Mueller, T., et al. Hum. Mol. Genet. 18(17):3334-3343(2009). Maier, B., et al. Genes Dev. 23(6):708-718(2009). Kang, H., et al. PLoS ONE 4 (8), E6611 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 6753540
• NCBI GeneID: 13000
• NCBI Accession #: NP_034104.1
• NCBI GenBank Nucleotide #: NM_009974.3
• UniProt Accession #: O54833
• Molecular Weight: 41,215 Da
• NCBI Official Full Name: casein kinase II subunit alpha'
• NCBI Official Synonym Full Names: casein kinase 2, alpha prime polypeptide
• NCBI Official Symbol: Csnk2a2
• NCBI Official Synonym Symbols: CK2; C77789; 1110035J23Rik
• NCBI Protein Information: casein kinase II subunit alpha'; CK II alpha'; casein kinase II, alpha 2, polypeptide
• UniProt Protein Name: Casein kinase II subunit alpha'
• Protein Family: Casein kinase
• UniProt Gene Name: Csnk2a2
• UniProt Synonym Gene Names: CK II alpha'
• UniProt Entry Name: CSK22_MOUSE

Uniprot Description

Function: Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins

By similarity.

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation: Constitutively active protein kinase whose activity is not directly affected by phosphorylation. Seems to be regulated by level of expression and localization

By similarity.

Subunit structure: Heterotetramer composed of two catalytic subunits (alpha chain and/or alpha' chain) and two regulatory subunits (beta chains). The tetramer can exist as a combination of 2 alpha/2 beta, 2 alpha'/2 beta or 1 alpha/1 alpha'/2 beta subunits. Also part of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, which forms following UV irradiation. Interacts with RNPS1

By similarity.

Disruption phenotype: Infertile male mice with oligospermia and globozoospermia. Ref.4

Miscellaneous: Can use both ATP and GTP as phosphoryl donors. Phosphorylation by casein kinase 2 has been estimated to represent up to one quarter of the eukaryotic phosphoproteome.

Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.Contains 1 protein kinase domain.