CD66b (CEACAM8) Monoclonal Antibody | anti-CD66b antibody

Anti-Human CD66b (CEACAM8)

Cat. Num. AAA666893

• Gene Names: CEACAM8; CD67; CGM6; CD66b; NCA-95
• Applications: Flow Cytometry, Functional Assay, ELISA
• Purity: Protein A purified antibody from tissue culture supernatant was reacted with FITC. Unconjugated FITC was separated from antibody/FITC conjugate by desalting column. The antibody/FITC conjugate is at approximately 0.5 mg/ml with a Fluorescein/IgG molar rat
• Synonyms: CD66b (CEACAM8); Monoclonal Antibody; Anti-Human CD66b (CEACAM8); anti-CD66b (ANC3C5)FITC Conjugate; Monoclonal anti-human CD66b (CEACAM8)/FITC*; anti-CD66b antibody

• Clonality: Monoclonal
• Isotype: IgG1K
• Clone Number: ANC3C5
• Purity/Purification: Protein A purified antibody from tissue culture supernatant was reacted with FITC. Unconjugated FITC was separated from antibody/FITC conjugate by desalting column. The antibody/FITC conjugate is at approximately 0.5 mg/ml with a Fluorescein/IgG molar rat
• Form/Format: FITC
• Sequence Length: 349

• Applicable Applications for anti-CD66b antibody: Flow Cytometry (FC/FACS), ELISA (EIA)
• Immunogen: Recombinant soluble human CD66b
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Performance: Five x 105 Polymorphprep prepared human Granulocytes(PMN) per tube were pelleted and pre incubated 5 minutes with 20ul of human IgG at 300ug/ml (to reduce non specific binding) after which they were washed and incubated 45 minutes on ice with 80 ul of anti-CD66b(ANC3C5) /FITC at a 1:50 dilution (10 ug/ml). They were then washed three times, fixed and analyzed by FACS. A 53% sub population of the cells stained positive with a mean shift of 1.22 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD66b(ANC3C5) antibody.
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Protect from light.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Flow Cytometry (FC/FACS)

Related Product Information for anti-CD66b antibody

Human CD66b (CEACAM8, CGMb, NCA-95, CD67) is a 95kD member of the Carcinoembryonic Antigen (CEA) group. It is expressed on granulocytes and functions as an adhesion molecule. Similar to the CD66 a, c, and d isoforms, engagement of CD66b on neutrophils causes increased adhesion to endothelial cells (2). Crosslinking CD66b on neutrophils with antibodies triggers IL-8 release (3).

Product Categories/Family for anti-CD66b antibody

Antibodies and Conjugates

References

1) W.N. Khan, S. Hammarstrom, et al. (1992) Genomics 14(2):384-90. 2) K.M. Skubitz, A.P. Skubitz, et al. (1996) J Leuk Biol 60(1): 106-117. 3) A.K. Schroder, L Rink, et al. (2006) Human Immunol 67(9): 676-682.

NCBI and Uniprot Product Information

• NCBI GI #: 21314600
• NCBI GeneID: 1088
• NCBI Accession #: NP_001807.2
• NCBI GenBank Nucleotide #: NM_001816.3
• Molecular Weight: 38,154 Da
• NCBI Official Full Name: carcinoembryonic antigen-related cell adhesion molecule 8
• NCBI Official Synonym Full Names: carcinoembryonic antigen related cell adhesion molecule 8
• NCBI Official Symbol: CEACAM8
• NCBI Official Synonym Symbols: CD67; CGM6; CD66b; NCA-95
• NCBI Protein Information: carcinoembryonic antigen-related cell adhesion molecule 8
• UniProt Protein Name: Carcinoembryonic antigen-related cell adhesion molecule 8
• UniProt Gene Name: CEACAM8
• UniProt Synonym Gene Names: CGM6
• UniProt Entry Name: CEAM8_HUMAN

Uniprot Description

CEACAM8: Belongs to the immunoglobulin superfamily. CEA family.

Protein type: Membrane protein, integral; Membrane protein, GPI anchor; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: protein binding

Biological Process: immune response; leukocyte migration