Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Spleen Tissue; Primary Ab: 10?g/ml Rabbit Anti-Human HPA Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Human Heparanase (HPSE) Polyclonal Antibody | anti-HPSE antibody
Polyclonal Antibody to Heparanase (HPSE)
Gene Names
HPSE; HPA; HPA1; HPR1; HSE1; HPSE1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Heparanase (HPSE); Polyclonal Antibody; Polyclonal Antibody to Heparanase (HPSE); HPA; HPR1; HPSE1; HSE1; Endo-glucoronidase;; anti-HPSE antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against HPSE. It has been selected for its ability to recognize HPSE in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Concentration
500ug/ml (varies by lot)
Sequence Length
543
Applicable Applications for anti-HPSE antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.2-2ug/mL; 1:250-2500
IHC: 5-20ug/mL; 1:25-100
ICC: 5-20ug/mL; 1:25-100
IHC: 5-20ug/mL; 1:25-100
ICC: 5-20ug/mL; 1:25-100
Cross Reactivity
Human
Conjugation
None
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Spleen Tissue; Primary Ab: 10?g/ml Rabbit Anti-Human HPA Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Spleen Tissue; Primary Ab: 10?g/ml Rabbit Anti-Human HPA Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Colon Tissue; Primary Ab: 10?g/ml Rabbit Anti-Human HPA AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Colon Tissue; Primary Ab: 10?g/ml Rabbit Anti-Human HPA AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Product Categories/Family for anti-HPSE antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
heparanase
NCBI Official Synonym Full Names
heparanase
NCBI Official Symbol
HPSE
NCBI Official Synonym Symbols
HPA; HPA1; HPR1; HSE1; HPSE1
NCBI Protein Information
heparanase
Protein Family
NCBI Description
Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Research Articles on HPSE
Similar Products
Product Notes
The HPSE (Catalog #AAA2111967) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Heparanase (HPSE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Heparanase (HPSE) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.2-2ug/mL; 1:250-2500 IHC: 5-20ug/mL; 1:25-100 ICC: 5-20ug/mL; 1:25-100. Researchers should empirically determine the suitability of the HPSE for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heparanase (HPSE), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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