Rabbit ADORA2A Polyclonal Antibody | anti-ADORA2A antibody

ADORA2A Polyclonal Antibody

Cat. Num. AAA127033

• Gene Names: ADORA2A; A2aR; RDC8; ADORA2
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: ADORA2A; Polyclonal Antibody; ADORA2A Polyclonal Antibody; ADORA2; RDC8; hA2Ar; anti-ADORA2A antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Sequence Length: 412

• Applicable Applications for anti-ADORA2A antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:1000; IF: 1:50 - 1:200
• Species Reactivity/Cross-Reactivity: Human, Mouse, Rat
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human ADORA2A (NP_000666.2).
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ADORA2A antibody (MBS127033) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Immunofluorescence (IF)

(Immunofluorescence analysis of C6 cells using ADORA2A antibody (MBS127033) at dilution of 1:100. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells using ADORA2A antibody (MBS127033) at dilution of 1:100. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence analysis of NIH/3T3 cells using ADORA2A antibody (mbs127033) at dilution of 1:100. Blue: DAPI for nuclear staining.)

Related Product Information for anti-ADORA2A antibody

Background: This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein, an adenosine receptor of A2A subtype, uses adenosine as the preferred endogenous agonist and preferentially interacts with the G(s) and G(olf) family of G proteins to increase intracellular cAMP levels. It plays an important role in many biological functions, such as cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. It has been implicated in pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. Alternative splicing results in multiple transcript variants. A read-through transcript composed of the upstream SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding.

Product Categories/Family for anti-ADORA2A antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 543740
• NCBI GeneID: 135
• UniProt Accession #: P29274; B2R7E0
• Molecular Weight: Observed MW: 45kDa; Calculated MW: 44kDa
• NCBI Official Full Name: Adenosine receptor A2a
• NCBI Official Synonym Full Names: adenosine A2a receptor
• NCBI Official Symbol: ADORA2A
• NCBI Official Synonym Symbols: A2aR; RDC8; ADORA2
• NCBI Protein Information: adenosine receptor A2a; adenosine receptor subtype A2a
• UniProt Protein Name: Adenosine receptor A2a
• Protein Family: Adenosine receptor
• UniProt Gene Name: ADORA2A
• UniProt Synonym Gene Names: ADORA2
• UniProt Entry Name: AA2AR_HUMAN

NCBI Description

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein, an adenosine receptor of A2A subtype, uses adenosine as the preferred endogenous agonist and preferentially interacts with the G(s) and G(olf) family of G proteins to increase intracellular cAMP levels. It plays an important role in many biological functions, such as cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. It has been implicated in pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. Alternative splicing results in multiple transcript variants. A read-through transcript composed of the upstream SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding. [provided by RefSeq, Jun 2013]

Uniprot Description

ADORA2A: one of several receptor subtypes for adenosine. A G-protein coupled receptor. Activation is mediated by G proteins which activate adenylyl cyclase. Abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.

Protein type: Membrane protein, integral; GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: membrane; integral to plasma membrane; plasma membrane

Molecular Function: identical protein binding; protein binding; enzyme binding; adenosine receptor activity, G-protein coupled

Biological Process: central nervous system development; nerve growth factor receptor signaling pathway; blood circulation; apoptosis; adenylate cyclase activation; phagocytosis; G-protein signaling, coupled to cAMP nucleotide second messenger; cell-cell signaling; sensory perception; cellular defense response; cAMP biosynthetic process; inflammatory response; blood coagulation; adenosine receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway