Rabbit MAOA Polyclonal Antibody | anti-MAOA antibody

MAOA (P347) Polyclonal Antibody

Cat. Num. AAA3003892

• Gene Names: MAOA; BRNRS; MAO-A
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MAOA; Polyclonal Antibody; MAOA (P347) Polyclonal Antibody; Amine oxidase [flavin-containing] A; Monoamine oxidase type A; MAO-A; anti-MAOA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MAO-A (P347) polyclonal antibody detects endogenous levels of MAO-A protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 527

• Applicable Applications for anti-MAOA antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 330-380 of Human MAOA.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MAOA (P347) pAb at 1:500 dilutionLane1:The Testis tissue lysate of Mouse (40ug) Lane2:The Testis tissue lysate of Rat (40ug) Lane3:The Kidney tissue lysate of Mouse (40ug) Lane4:HCT116 whole cell lysate (40ug) Lane5:Hela whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of MAO-A (P347) pAb in paraffin-embedded human brain tissue.)

Related Product Information for anti-MAOA antibody

Monoamine oxidase (MAO) is an enzyme of the mitochondrial outer membrane and catalyzes the oxidative deamination of biogenic amines throughout the body. MAO is critical in the neuronal metabolism of catecholamine and indolamine transmitters. Cultured skin fibroblasts show both MAO-A and MAO-B and both MAOs differ in molecular structure. MAO-A, the primary type in fibroblasts, preferentially degrades serotonin and norepinephrine.

NCBI and Uniprot Product Information

• NCBI GI #: 113978
• NCBI GeneID: 4128
• UniProt Accession #: P21397
• Molecular Weight: ~ 60kDa
• NCBI Official Full Name: Amine oxidase
• NCBI Official Synonym Full Names: monoamine oxidase A
• NCBI Official Symbol: MAOA
• NCBI Official Synonym Symbols: BRNRS; MAO-A
• NCBI Protein Information: amine oxidase [flavin-containing] A
• UniProt Protein Name: Amine oxidase [flavin-containing] A
• Protein Family: Amine oxidase
• UniProt Gene Name: MAOA
• UniProt Synonym Gene Names: MAO-A
• UniProt Entry Name: AOFA_HUMAN

NCBI Description

This gene is one of two neighboring gene family members that encode mitochondrial enzymes which catalyze the oxidative deamination of amines, such as dopamine, norepinephrine, and serotonin. Mutation of this gene results in Brunner syndrome. This gene has also been associated with a variety of other psychiatric disorders, including antisocial behavior. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012]

Uniprot Description

MAOA: Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine. Defects in MAOA are the cause of Brunner syndrome (BRUNS). Brunner syndrome is a form of X-linked non- dysmorphic mild mental retardation. Male patients are affected by a syndrome of borderline mental retardation and exhibit abnormal behavior, including disturbed regulation of impulsive aggression. Obligate female carriers have normal intelligence and behavior. Belongs to the flavin monoamine oxidase family.

Protein type: EC 1.4.3.4; Membrane protein, integral; Amino Acid Metabolism - tryptophan; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Amino Acid Metabolism - histidine; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - glycine, serine and threonine; Oxidoreductase; Amino Acid Metabolism - tyrosine

Chromosomal Location of Human Ortholog: Xp11.3

Cellular Component: mitochondrial outer membrane; mitochondrion; integral to membrane

Molecular Function: amine oxidase activity

Biological Process: synaptic transmission; biogenic amine metabolic process; neurotransmitter catabolic process; dopamine catabolic process; xenobiotic metabolic process; neurotransmitter secretion; neurotransmitter biosynthetic process

Disease: Brunner Syndrome