Rabbit anti-Human, Mouse TRIAD3 Polyclonal Antibody | anti-TRIAD3 antibody

TRIAD3, CT (E3 Ubiquitin-protein Ligase RNF216, RING Finger Protein 216, Ubiquitin Conjugating Enzyme 7 Interacting Protein 1, Triad3 Protein, Zinc Finger Protein Inhibiting NF-kappa-B, Triad Domain-containing Protein 3, UBCE7IP1, RNF216, ZIN) (APC)

Cat. Num. AAA6503532

• Gene Names: RNF216; ZIN; CAHH; U7I1; TRIAD3; UBCE7IP1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: TRIAD3; Polyclonal Antibody; CT (E3 Ubiquitin-protein Ligase RNF216; RING Finger Protein 216; Ubiquitin Conjugating Enzyme 7 Interacting Protein 1; Triad3 Protein; Zinc Finger Protein Inhibiting NF-kappa-B; Triad Domain-containing Protein 3; UBCE7IP1; RNF216; ZIN) (APC); anti-TRIAD3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TRIAD3. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 488

• Applicable Applications for anti-TRIAD3 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human UBCE7IP1 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-UBCE7IP1 Pab is used in Western blot to detect UBCE7IP1 in mouse kidney tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-TRIAD3 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

1. Ota, T., et al., Nat. Genet. 36(1):40-45 (2004). 2. Chen, D., et al., J. Biol. Chem. 277(18):15985-15991 (2002). 3. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 37729466
• NCBI GeneID: 54476
• UniProt Accession #: Q9NWF9
• Molecular Weight: 56.697
• NCBI Official Full Name: E3 ubiquitin ligase TRIAD3C
• NCBI Official Synonym Full Names: ring finger protein 216
• NCBI Official Symbol: RNF216
• NCBI Official Synonym Symbols: ZIN; CAHH; U7I1; TRIAD3; UBCE7IP1
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF216
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF216
• UniProt Gene Name: RNF216
• UniProt Synonym Gene Names: TRIAD3; UBCE7IP1; ZIN
• UniProt Entry Name: RN216_HUMAN

NCBI Description

This gene encodes a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates. Several alternatively spliced transcript variants have been described for this locus but the full-length natures of only some are known. [provided by RefSeq, Jul 2008]

Uniprot Description

TRIAD3: Isoform 1 acts as an E3 ubiquitin ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Promotes degradation of TRAF3, TLR4 and TLR9. Contributes to the regulation of antiviral responses. Down- regulates activation of NF-kappa-B, IRF3 activation and IFNB production. Isoform 3/ZIN inhibits TNF and IL-1 mediated activation of NF-kappa-B. Promotes TNF and RIP mediated apoptosis. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.19; Ligase

Chromosomal Location of Human Ortholog: 7p22.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; zinc ion binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; viral reproduction; apoptosis; regulation of interferon-beta production; regulation of defense response to virus by host

Disease: Gordon Holmes Syndrome