Human LAIR1 ELISA Kit | LAIR1 elisa kit

Human LAIR1 ELISA Kit

Cat. Num. AAA1753890

• Gene Names: LAIR1; CD305; LAIR-1
• Reactivity: Human
• Synonyms: LAIR1; Human LAIR1 ELISA Kit; CD305; LAIR-1; leukocyte associated immunoglobulin like receptor 1; LAIR1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human LAIR1

• Samples: cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
• Detection Range: 62.5pg/ml-4,000pg/ml
• Sensitivity: <50pg/ml
• Capture/Detection Antibodies: Capture antibody- a polyclonal antibody from goat|Detection antibody- a biotinylated polyclonal antibody from goat
• Sample Data: Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000; O.D.|0.049|0.138|0.186|0.273|0.479|0.853|1.426|2.059
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|210|976|1958|; Standard deviation|11.34|67.34|140.98|; CV(%)|5.4%|6.9%|7.2%|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|216|989|1970; Standard deviation|15.55|84.07|181.24; CV(%)|7.2%|8.5%|9.2%
• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 210|208|212|206|209|12.96|6.2%; Sample 2: 976|962|985|967|973|65.19|6.7%; Sample 3: 1958|1930|1942|1964|1948.5|140.29|7.2%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: E Coli; Immunogen sequence: Q22-Y165
• Anticoagulant: heparin, EDTA or citrate
• Principle: Human LAIR1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for LAIR1 has been precoated onto 96-well plates. Standards(Expression system for standard: E Coli, Immunogen sequence: Q22-Y165) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LAIR1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse LAIR1 amount of sample captured in plate.
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human LAIR1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" for details. It is recommended that each human LAIR1 standard solution and each sample be measured in duplicate.
• TMB Incubation Time: 15-20
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Testing Data

Related Product Information for LAIR1 elisa kit

The protein encoded by this gene is an inhibitory receptor found on peripheral mononuclear cells, including natural killer cells, T cells, and B cells. Inhibitory receptors regulate the immune response to prevent lysis of cells recognized as self. The gene is a member of both the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family. The gene maps to a region of 19q13. 4 called the leukocyte receptor cluster, which contains at least 29 genes encoding leukocyte-expressed receptors of the immunoglobulin superfamily. The encoded protein has been identified as an anchor for tyrosine phosphatase SHP-1, and may induce cell death in myeloid leukemias. Alternative splicing results in multiple transcript variants.

Product Categories/Family for LAIR1 elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GI #: 612407867
• NCBI GeneID: 3903
• NCBI Accession #: NP_001275952.2
• NCBI GenBank Nucleotide #: NM_001289023.2
• UniProt Accession #: Q6GTX8
• Molecular Weight: 23,049 Da
• NCBI Official Full Name: leukocyte-associated immunoglobulin-like receptor 1 isoform c
• NCBI Official Synonym Full Names: leukocyte-associated immunoglobulin-like receptor 1
• NCBI Official Symbol: LAIR1
• NCBI Official Synonym Symbols: CD305; LAIR-1
• NCBI Protein Information: leukocyte-associated immunoglobulin-like receptor 1; leukocyte-associated Ig-like receptor 1
• UniProt Protein Name: Leukocyte-associated immunoglobulin-like receptor 1
• Protein Family: Leukocyte-associated immunoglobulin-like receptor
• UniProt Gene Name: LAIR1
• UniProt Synonym Gene Names: CD305; LAIR-1; hLAIR1
• UniProt Entry Name: LAIR1_HUMAN

NCBI Description

The protein encoded by this gene is an inhibitory receptor found on peripheral mononuclear cells, including natural killer cells, T cells, and B cells. Inhibitory receptors regulate the immune response to prevent lysis of cells recognized as self. The gene is a member of both the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family. The gene maps to a region of 19q13.4 called the leukocyte receptor cluster, which contains at least 29 genes encoding leukocyte-expressed receptors of the immunoglobulin superfamily. The encoded protein has been identified as an anchor for tyrosine phosphatase SHP-1, and may induce cell death in myeloid leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

LAIR-1: an inhibitory receptor found on peripheral mononuclear cells, including NK cells, T cells, and B cells. Inhibitory receptors regulate the immune response to prevent lysis of cells recognized as self. The gene is a member of both the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family. The gene maps to a region called the leukocyte receptor cluster, which contains at least 25 genes encoding leukocyte-expressed receptors of the immunoglobulin superfamily.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: plasma membrane; integral to membrane

Molecular Function: protein binding

Biological Process: immune system process