Mouse CD3 Monoclonal Antibody | anti-CD3 antibody
MOUSE ANTI CHICKEN CD3
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
Synonyms
CD3; Monoclonal Antibody; MOUSE ANTI CHICKEN CD3; anti-CD3 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
CT-3
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 0.5 mg/ml (varies by lot)
Sequence Length
175
Applicable Applications for anti-CD3 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
Flow Cytometry: Use 50ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Maximum Dilution: 1/50
Flow Cytometry: Maximum Dilution: 1/50
Preparation
Purified IgG prepared from ascites
Immunogen
Chicken thymocytes and Ig -ve peripheral blood mononuclear cells.
Fusion Partners
Fusion Partners
Target Species
Chicken
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Related Product Information for anti-CD3 antibody
Mouse anti Chicken CD3, clone CT-3 recognizes the chicken CD3 cell surface antigen, a complex of at least three polypeptides of 20, 19 and 17kDa expressed by T cells (Chen et al. 1986). The antibody also co-precipitates a 90kDa heterodimer from digitonin solubilized T cell lysates, which can be reduced to two polypeptides of 40kDa and 50 kD. In the chicken, CD3 is expressed by approximately 30% of thymocytes, 70% of peripheral blood mononuclear cells, but
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
19,062 Da
NCBI Official Full Name
T-cell surface glycoprotein CD3 epsilon chain
UniProt Protein Name
T-cell surface glycoprotein CD3 epsilon chain
Protein Family
UniProt Gene Name
CD3E
UniProt Entry Name
CD3E_CHICK
Uniprot Description
The CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. Required for normal immune responses.
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Product Notes
The CD3 cd3e (Catalog #AAA212164) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD3 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP). Flow Cytometry: Use 50ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Maximum Dilution: 1/50. Researchers should empirically determine the suitability of the CD3 cd3e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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