AREG recombinant protein

AREG

Cat. Num. AAA516579

• Gene Names: AREG; AR; SDGF; CRDGF
• Applications: Cell Assay
• Synonyms: AREG; AR; Colorectum cell-derived growth factor; CRDGF Recombinant Human Amphiregulin (AREG).; AREG recombinant protein

• Host: E Coli
• Form/Format: Lyophilized from a 0.2 um filtered solution in PBS with BSA as a carrier protein

• Applicable Applications for AREG recombinant protein: Cell Assay
• Species: Human
• Endotoxin Level: <1.0EU/ug of recombinant protein as determined by the LAL method.
• Reconstitution: Reconstitute at 100ug/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at -70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Activity

(Sample Cell Assay Data: Measured in a cell proliferation assay using mouse embryonic fibroblast 3T3 cells. The ED(50) for this effect is )

SDS-Page

(Purity: Recombinant Human AREG resolved on a 17% SDS_PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250.)

Related Product Information for AREG recombinant protein

Recombinant Human AREG was expressed in E Coli cells. The protein accession number is P15514
Amphiregulin is a trans-membrane protein whose protease mediated digestion of the extracellular domain enables release of mature AR. Amphiregulin works to stimulate the proliferation of some animal keratinocyles, epithelial cells and fibroblasts. It has been shown that the mRNA expression of amphiregulin can be detected in numerous carcinoma cell lines and the epithelial cells of various human tissues, including colon, stomach, breast, ovary and kidney.

Product Categories/Family for AREG recombinant protein

Growth Factors; Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 4502199
• NCBI GeneID: 374
• NCBI Accession #: NP_001648.1
• NCBI GenBank Nucleotide #: NM_001657.2
• UniProt Accession #: P15514
• Molecular Weight: 27,895 Da
• NCBI Official Full Name: amphiregulin preproprotein
• NCBI Official Synonym Full Names: amphiregulin
• NCBI Official Symbol: AREG
• NCBI Official Synonym Symbols: AR; SDGF; CRDGF
• NCBI Protein Information: amphiregulin; schwannoma-derived growth factor; colorectum cell-derived growth factor
• UniProt Protein Name: Amphiregulin
• Protein Family: Amphiregulin
• UniProt Gene Name: AREG
• UniProt Synonym Gene Names: SDGF; AR; CRDGF
• UniProt Entry Name: AREG_HUMAN

NCBI Description

The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells, and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). This protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells and inhibits the growth of certain aggressive carcinoma cell lines. This encoded protein is associated with a psoriasis-like skin phenotype. [provided by RefSeq, Jul 2008]

Uniprot Description

AREG: Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts. Belongs to the amphiregulin family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; cell surface; cytoplasm; integral to membrane; nucleus

Molecular Function: protein binding; growth factor activity; cytokine activity; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; response to peptide hormone stimulus; response to cAMP; response to glucocorticoid stimulus; response to estradiol stimulus; glial cell proliferation; G-protein coupled receptor protein signaling pathway; cell proliferation; cell-cell signaling; response to hydrogen peroxide; positive regulation of cell proliferation; negative regulation of osteoblast differentiation; neurite development; positive regulation of DNA replication; positive regulation of phosphorylation