anti-Human, Mouse Alpha-catenin Antibody | anti-CTNNA1 antibody

Alpha-catenin (Ab-641) antibody

Cat. Num. AAA857401

• Gene Names: CTNNA1; MDPT2; CAP102
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: Alpha-catenin; Alpha-catenin (Ab-641) antibody; anti-CTNNA1 antibody

• Reactivity: Human, Mouse
• Specificity: alpha-catenin (Ab-641) antibody detects endogenous levels of total alpha-catenin protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 841

• Applicable Applications for anti-CTNNA1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human alpha-catenin around the phosphorylation site of serine 641 (D-D-SP-D-F).
• Preparation and Storage: Store at-20 degree C for 1 year

Western Blot (WB)

(Western blot analysis of extract from A431 cells untreated or treated with EGF (200ng/ml, 30min), using alpha-catenin (Ab-641) antibody and alpha-catenin (Phospho-Ser641) antibody.)

Product Categories/Family for anti-CTNNA1 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Hwang, S.G. et al. (2005) J. Biol. Chem. 280, 12758-12765 Drees, F. et al. (2005) Cell 123, 903-915. Yamada, S. et al. (2005) Cell 123, 889-901. Kobielak, A. and Fuchs, E. (2004) Nat. Rev. Mol. Cell Biol. 5, 614-625.

NCBI and Uniprot Product Information

• NCBI GI #: 594140506
• NCBI GeneID: 1495
• NCBI Accession #: NP_001277236.1
• NCBI GenBank Nucleotide #: NM_001290307.2
• UniProt Accession #: P35221; Q12795; Q8N1C0
• Molecular Weight: 59,550 Da
• NCBI Official Full Name: catenin alpha-1 isoform 2
• NCBI Official Synonym Full Names: catenin alpha 1
• NCBI Official Symbol: CTNNA1
• NCBI Official Synonym Symbols: MDPT2; CAP102
• NCBI Protein Information: catenin alpha-1
• UniProt Protein Name: Catenin alpha-1
• Protein Family: Alpha-catenin-like protein
• UniProt Gene Name: CTNNA1

NCBI Description

This gene encodes a member of the catenin family of proteins that play an important role in cell adhesion process by connecting cadherins located on the plasma membrane to the actin filaments inside the cell. The encoded mechanosensing protein contains three vinculin homology domains and undergoes conformational changes in response to cytoskeletal tension, resulting in the reconfiguration of cadherin-actin filament connections. Certain mutations in this gene cause butterfly-shaped pigment dystrophy. [provided by RefSeq, May 2016]

Uniprot Description

Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.