Testing Data
(Staining of rat lymph node cells with Mouse anti Rat CD5: FITC (MBS211051))
Mouse CD5 Monoclonal Antibody | anti-CD5 antibody
MOUSE ANTI RAT CD5
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry
Synonyms
CD5; Monoclonal Antibody; MOUSE ANTI RAT CD5; anti-CD5 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
OX-19
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Sequence Length
491
Applicable Applications for anti-CD5 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP), Immunohistology Paraffin*
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Maximum Dilution: 1/100
Immunohistology - Paraffin: Application Note: The use of PLP fixation is recommended for optimal results.
Flow Cytometry: Maximum Dilution: 1/100
Immunohistology - Paraffin: Application Note: The use of PLP fixation is recommended for optimal results.
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Preparation
Immunogen
Rat thymocyte glycoproteins
Fusion Partners
Fusion Partners
Target Species
Rat
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data
(Staining of rat lymph node cells with Mouse anti Rat CD5: FITC (MBS211051))
Testing Data
(Staining of rat lymph node cells with Mouse anti Rat CD5: FITC (MBS211051))
Testing Data
(Staining of rat splenocytes with Mouse anti Rat CD5:FITC )
Testing Data
(Staining of rat splenocytes with Mouse anti Rat CD5:FITC )
Related Product Information for anti-CD5 antibody
Mouse anti Rat CD5 antibody, clone OX-19 recognizes the rat CD5 cell surface antigen, a 69kD glycoprotein expressed by T cells, thymocytes and a subset of B cells. Mouse anti Rat CD5 antibody, clone OX-19 has been reported as being suitable for use on periodate-lysine paraformaldehyde (PLP) fixed paraffin embedded tissue (Whiteland et al. 1995). Mouse anti Rat CD5 antibody, clone OX-19 is routinely tested in flow cytometry on rat splenocytes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,440 Da
NCBI Official Full Name
T-cell surface glycoprotein CD5
NCBI Official Synonym Full Names
Cd5 molecule
NCBI Official Symbol
Cd5
NCBI Protein Information
T-cell surface glycoprotein CD5; CD5 antigen; Lymphocyte antigen CD5
UniProt Protein Name
T-cell surface glycoprotein CD5
Protein Family
UniProt Gene Name
Cd5
UniProt Entry Name
CD5_RAT
NCBI Description
binds CD2 glycoprotein on thymocytes; may play a role in T lymphocyte signal transduction [RGD, Feb 2006]
Uniprot Description
CD5: a surface glycoprotein expressed on T cells and B-1 B cells. Rapidly recruited to the immunological synapse, lowering the response of the T cell antigen receptor. CD5 interacts with CD72/LYB-2. Promotes B-cell survival through stimulation of autocrine IL-10 production. Contains 3 SRCR domains.
Protein type: Membrane protein, integral; Cell surface
Cellular Component: membrane; plasma membrane; external side of plasma membrane
Molecular Function: glycoprotein binding; scavenger receptor activity
Biological Process: receptor-mediated endocytosis; T cell costimulation; regulation of signal transduction
Research Articles on CD5
Similar Products
Product Notes
The CD5 cd5 (Catalog #AAA213072) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD5 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP), Immunohistology Paraffin*. Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Maximum Dilution: 1/100 Immunohistology - Paraffin: Application Note: The use of PLP fixation is recommended for optimal results. Researchers should empirically determine the suitability of the CD5 cd5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
