Immunofluorescence (IF)
(Immunofluorescence staining of fixed HeLa cells expressing mCherry tagged GCN4 with anti-GCN4 antibody C11L34 Immunofluorescence analysis of fixed HeLa cells stained with the chimeric rabbit IgG version of C11L34 followed by an Alexa Fluor 488 secondary antibody, showing mitochondrial staining. Colocalisation with mCherry tagged GCN4 demonstrates the very low off target/non-specific binding of this antibody. The nuclear and mitochondiral DNA stain is hoechst (blue). Panels show; a) mCherry tagged, mitochondrially targetted GCN4, b) with Alexa Fluor 488 secondary antibody, c) DNA stained with hoechst, d) Merged channels. Images courtesy of Luming Chen (Kim Orth lab, UT Southwestern Medical Center).)
GCN4 recombinant antibody
Anti-GCN4 [C11L34]
Immunofluorescence (IF)
(Immunofluorescence staining of fixed HeLa cells expressing mCherry tagged GCN4 with anti-GCN4 antibody C11L34 Immunofluorescence analysis of fixed HeLa cells stained with the chimeric rabbit IgG version of C11L34 followed by an Alexa Fluor 488 secondary antibody, showing mitochondrial staining. Colocalisation with mCherry tagged GCN4 demonstrates the very low off target/non-specific binding of this antibody. The nuclear and mitochondiral DNA stain is hoechst (blue). Panels show; a) mCherry tagged, mitochondrially targetted GCN4, b) with Alexa Fluor 488 secondary antibody, c) DNA stained with hoechst, d) Merged channels. Images courtesy of Luming Chen (Kim Orth lab, UT Southwestern Medical Center).)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed HeLa cells expressing mCherry tagged GCN4 with anti-GCN4 antibody C11L34 Immunofluorescence analysis of fixed HeLa cells stained with the chimeric rabbit IgG version of C11L34 followed by an Alexa Fluor 488 secondary antibody, showing mitochondrial staining. Colocalisation with mCherry tagged GCN4 demonstrates the very low off target/non-specific binding of this antibody. The nuclear and mitochondiral DNA stain is hoechst (blue). Panels show; a) mCherry tagged, mitochondrially targetted GCN4, b) with Alexa Fluor 488 secondary antibody, c) DNA stained with hoechst, d) Merged channels. Images courtesy of Luming Chen (Kim Orth lab, UT Southwestern Medical Center).)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed HeLa cells expressing mCherry tagged GCN4 with anti-GCN4 antibody C11L34 Immunofluorescence analysis of fixed HeLa cells stained with the chimeric mouse IgG1 version of C11L34 (MBS488425) followed by an Alexa Fluor 488 secondary antibody, showing mitochondrial staining. Colocalisation with mCherry tagged GCN4 demonstrates the very low off target/non-specific binding of this antibody. The nuclear and mitochondiral DNA stain is DAPI (blue). Panels show; a) mCherry tagged, mitochondrially targetted GCN4, b) MBS488425 with Alexa Fluor 488 secondary antibody, c) DNA stained with DAPI, d) Merged channels. Images courtesy of Luming Chen (Kim Orth lab, UT Southwestern Medical Center).)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed HeLa cells expressing mCherry tagged GCN4 with anti-GCN4 antibody C11L34 Immunofluorescence analysis of fixed HeLa cells stained with the chimeric mouse IgG1 version of C11L34 (MBS488425) followed by an Alexa Fluor 488 secondary antibody, showing mitochondrial staining. Colocalisation with mCherry tagged GCN4 demonstrates the very low off target/non-specific binding of this antibody. The nuclear and mitochondiral DNA stain is DAPI (blue). Panels show; a) mCherry tagged, mitochondrially targetted GCN4, b) MBS488425 with Alexa Fluor 488 secondary antibody, c) DNA stained with DAPI, d) Merged channels. Images courtesy of Luming Chen (Kim Orth lab, UT Southwestern Medical Center).)
Original Published Journal: Journal of Biological Chemistry 2004; 279(18):18870-18877
Original Published Journal: Journal of Biological Chemistry 2004; 279(18):18870-18877
Publication Note: Describes the generation of an scFv with high affinity using directed evolution.
NCBI and Uniprot Product Information
Uniprot Description
Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'.
Research Articles on GCN4
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Product Notes
The GCN4 gcn4 (Catalog #AAA488425) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-GCN4 [C11L34] reacts with S. cerevisiae and may cross-react with other species as described in the data sheet. AAA Biotech's GCN4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Surface Plasmon Resonance (SPR), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the GCN4 gcn4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GCN4, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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