Mouse anti-Bovine CD28 Monoclonal Antibody | anti-CD28 antibody

Mouse Anti Bovine CD28

Cat. Num. AAA225673

• Reactivity: Bovine
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified IgG prepared by affinity chromatography on Protein A
• Synonyms: CD28; Monoclonal Antibody; Mouse Anti Bovine CD28; CD28 antibody (CC219); anti-CD28 antibody

• Host: Mouse
• Reactivity: Bovine
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: CC219
• Purity/Purification: Purified IgG prepared by affinity chromatography on Protein A
• Form/Format: Liquid; Phosphate Buffered Saline
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 219

• Applicable Applications for anti-CD28 antibody: Flow Cytometry (FC/FACS)
• Immunogen: Plasmid DNA encoding the bovine CD28 gene
• Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the SP/2/0 myeloma cell line.
• Preservative Stabilizers: 0.09% Sodium Azide
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred.; Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.; Should this product contain a precipitate we recommend microcentrifugation before use.

Testing Data

(Figure A. FITC conjugated Mouse anti Bovine CD4 and RPE conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Bovine CD4 and RPE conjugated Mouse anti Bovine CD28 . All experiments performed on red cell lysed bovine blood gated on lymphocytes in the presence of 10% bovine serum. Data acquired on the ZE5 Cell Analyzer.)

Testing Data

(Figure A. FITC conjugated Mouse anti Bovine CD44 and RPE conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Bovine CD44 and RPE conjugated Mouse anti Bovine CD28 . All experiments performed on red cell lysed bovine blood gated on mononuclear cells.)

Testing Data

(Figure A. RPE conjugated Mouse anti Bovine CD28 and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Bovine CD28 and FITC conjugated Mouse anti Bovine CD44 . All experiments performed on red cell lysed bovine blood gated on mononuclear cells.)

Related Product Information for anti-CD28 antibody

Mouse anti Bovine CD28, clone CC219, recognizes the bovine homologue of human CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). CD28 is involved in increased production of IL-4 and IL-10 in T cells.
Mouse anti Bovine CD28, clone CC219 is able to stimulate bovine T cells in combination with anti-bovine CD3. For functional studies it is recommended to use the low endotoxin and preservative free format of this antibody, MCA5779EL.
Mouse anti Bovine CD28, clone CC219 has previously been published under the alternative clone designation F848EC4 (Hogg et al. 2011).

NCBI and Uniprot Product Information

• NCBI GI #: 30794304
• NCBI GeneID: 281050
• NCBI Accession #: NP_851347.1
• NCBI GenBank Nucleotide #: NM_181004.1
• UniProt Accession #: Q28071
• NCBI Official Full Name: T-cell-specific surface glycoprotein CD28
• NCBI Official Synonym Full Names: CD28 molecule
• NCBI Official Symbol: CD28
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• Protein Family: T-cell-specific surface glycoprotein
• UniProt Gene Name: CD28
• UniProt Entry Name: CD28_BOVIN

Uniprot Description

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation.