Mouse CD28 Monoclonal Antibody | anti-CD28 antibody

MOUSE ANTI BOVINE CD28:FITC

Cat. Num. AAA224710

• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD28; Monoclonal Antibody; MOUSE ANTI BOVINE CD28:FITC; anti-CD28 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: CC219
• Form/Format: FITC; Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration: Ig concentration 0.1mg/ml (varies by lot)
• Sequence Length: 219

• Applicable Applications for anti-CD28 antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin; Preparation
• Immunogen: Plasmid DNA encoding the bovine CD28 gene; Fusion Partners
• Target Species: Bovine
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Bovine PBMCs stained with Mouse anti Bovine CD28 (MBS224419) followed by Goat anti Mouse IgG:FITC (MBS235142))

Testing Data #2

(Bovine PBMCs stained with Mouse anti Bovine CD28:FITC (MBS224710))

Related Product Information for anti-CD28 antibody

Mouse anti Bovine CD28, clone CC219, recognizes the bovine homologue of human CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). Clone CC219 has been shown to be able to stimulate bovine T lymphocytes in combination with anti-bovine CD3. For functional studies it is recommended to use the low endotoxin and preservative free format of this antibody, MBS224759.Clone CC219 has previously been published under the alternative clone designation F848EC4.

NCBI and Uniprot Product Information

• NCBI GI #: 30794304
• NCBI GeneID: 281050
• NCBI Accession #: NP_851347.1
• NCBI GenBank Nucleotide #: NM_181004.1
• UniProt Accession #: Q28071
• Molecular Weight: 25,143 Da
• NCBI Official Full Name: T-cell-specific surface glycoprotein CD28
• NCBI Official Synonym Full Names: CD28 molecule<
• NCBI Official Symbol: CD28
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28; antigen CD28
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• Protein Family: T-cell-specific surface glycoprotein
• UniProt Gene Name: CD28
• UniProt Entry Name: CD28_BOVIN

Uniprot Description

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation.