Mouse anti-Human, Pig CD54 Monoclonal Antibody | anti-CD54 antibody

anti-human CD54 (D1)-R-PE

Cat. Num. AAA666659

• Reactivity: Human, Pig
• Applications: Flow Cytometry, Functional Assay, Western Blot, ELISA
• Synonyms: CD54; Monoclonal Antibody; anti-human CD54 (D1)-R-PE; Monoclonal anti-human CD54 (ICAM-1)/R-PE; anti-CD54 antibody

• Host: Mouse
• Reactivity: Human, Pig
• Clonality: Monoclonal
• Isotype: IgG1k
• Clone Number: 15.2
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA.
• Concentration: Volume: 0.2ml (varies by lot)

• Applicable Applications for anti-CD54 antibody: Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA), FX
• Application Notes: 1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test)
• Performance: Five x 105 cultured human Raji cells per tube were washed and pre incubated 5 minutes with 20ul of 250ug/ml human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-CD54/R-PE at a dilution factor of 1:50 (5 ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive (93% net) with a mean shift of 2.26 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti- CD54 antibody.
• Conjugation: PE
• Production: Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 0.25 mg/ml with an A565/A280 ratio of 2.85.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: Human monocytes
• Preparation and Storage: Store at 2 - 5 degree C. Do not freeze! Protect from light.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD54 antibody

Information: Human CD54 (ICAM-1) mediates cell adhesion by binding to the integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). CD54 expression on resting peripheral blood leukocytes in weak but is upregulated on activated T and B lymphocytes and on monocytes.Antibody 15.2 recognizes the D1 domain of the CD54 molecule of 90 kd. Antibody 15.2 inhibits CD54 binding to LFA-1.

References

A.R. Berendt, et al, (1992) Cell 68: 71-81. I. Dransfield, et al, (1992) J Cell Biol 116: 1527-1535. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1548-1550. P.L. Reilly, et al, (1995) J Immunol 155: 529-532.