Typical Testing Data/Standard Curve (for reference only)
CXCL10 / IP-10 elisa kit
CXCL10 / IP-10 ELISA Kit
Synonyms
CXCL10 / IP-10; CXCL10 / IP-10 ELISA Kit; C-X-C motif chemokine 10; CXCL10; Interferon gamma-induced protein 10; IP-10; small-inducible cytokine B10; CXCL10 / IP-10 elisa kit
Specificity
A sample panel of in all n = 327 stool specimens from a routine microbiological laboratory was tested in the Cryptosporidium parvum ELISA: Negative: n = 325 · Positive: n = 2 · Specificity: 99.4% The two ELISA positive samples were confirmed true positive by direct immunofluorescence: Specificity amended: 100% for this sample panel.
Samples
Faecal Samples
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CXCL10 / IP-10 elisa kit
Intended Uses: The Cryptosporidium parvum ELISA is an enzyme immunoassay for the measurement of Cryptosporidium parvum in faecal samples. For Research Use Only..
Principle of the Assay: The Cryptosporidium parvum is based on monoclonal antibodies to Cryptosporidium parvum specific proteins. Within the first incubation step (60 min, room temperature, RT) diluted stool specimens as well as positive and negative controls react with the solid-phase adsorbed antibodies. Unbound components are removed by a subsequent washing step. Within the second incubation step (30 min, RT) solid-phase bound immune complexes react with the horseradish (HRP)-labelled antibodies of the conjugate. Unbound reagents are separated from the solid-phase antibody-antigen-antibody immune complexes by a further washing step. HRP converts the subsequently added colourless chromogenic substrate solution into a blue product. The enzyme reaction is terminated by sulphuric acid dispensed into the wells after 10 min incubation at RT turning the solution from blue to yellow. The optical density (OD) is read at 450 / >= 620 nm with a microplate photometer!!Background/Introduction: The genus Cryptosporidium belongs to the phylum Apicomplexa, protozoan parasites characterized by secretory cell organells enabling the invasion of host cells. Among different human pathogenic species Cryptosporidium parvum represents the most important cause of diarrhea in man but also in several animal species (Zoonosis) (1, 2). The transmission of Cryptosporidium is effected by food or drinking water contaminated with faeces. Person to person transmission has also been described. After ingestion of the infective stage, the oocyst, the vegetative motile sporozoites will be released in the small intestine, where they invade into the enterocytes and stay in a parasitophorous vacuole beneath the microvilli. The sporozoites multiply by bisection (asexually) as well as sexually. In the course of sexual multiplication micro-and macrogamonts emerge and fuse to form the infective oocyst. The oocysts can be devided into thin-wall and thick-wall oocysts: the thin-wall oocysts are responsible for autoinfections within the same host whereas the thick-wall oocysts are excreted with the faeces and are therefore responsible for transmission to other hosts. Cryptosporidium infections are usually determined by direct pathogen detection from faecal specimens either by microscopy of dyed smears, immune fluorescence microscopy or by immunological methods like ELISA. Nucleic acid amplification techniques (PCR) are also established but still restricted to special laboratories.
Principle of the Assay: The Cryptosporidium parvum is based on monoclonal antibodies to Cryptosporidium parvum specific proteins. Within the first incubation step (60 min, room temperature, RT) diluted stool specimens as well as positive and negative controls react with the solid-phase adsorbed antibodies. Unbound components are removed by a subsequent washing step. Within the second incubation step (30 min, RT) solid-phase bound immune complexes react with the horseradish (HRP)-labelled antibodies of the conjugate. Unbound reagents are separated from the solid-phase antibody-antigen-antibody immune complexes by a further washing step. HRP converts the subsequently added colourless chromogenic substrate solution into a blue product. The enzyme reaction is terminated by sulphuric acid dispensed into the wells after 10 min incubation at RT turning the solution from blue to yellow. The optical density (OD) is read at 450 / >= 620 nm with a microplate photometer!!Background/Introduction: The genus Cryptosporidium belongs to the phylum Apicomplexa, protozoan parasites characterized by secretory cell organells enabling the invasion of host cells. Among different human pathogenic species Cryptosporidium parvum represents the most important cause of diarrhea in man but also in several animal species (Zoonosis) (1, 2). The transmission of Cryptosporidium is effected by food or drinking water contaminated with faeces. Person to person transmission has also been described. After ingestion of the infective stage, the oocyst, the vegetative motile sporozoites will be released in the small intestine, where they invade into the enterocytes and stay in a parasitophorous vacuole beneath the microvilli. The sporozoites multiply by bisection (asexually) as well as sexually. In the course of sexual multiplication micro-and macrogamonts emerge and fuse to form the infective oocyst. The oocysts can be devided into thin-wall and thick-wall oocysts: the thin-wall oocysts are responsible for autoinfections within the same host whereas the thick-wall oocysts are excreted with the faeces and are therefore responsible for transmission to other hosts. Cryptosporidium infections are usually determined by direct pathogen detection from faecal specimens either by microscopy of dyed smears, immune fluorescence microscopy or by immunological methods like ELISA. Nucleic acid amplification techniques (PCR) are also established but still restricted to special laboratories.
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Product Notes
The CXCL10 / IP-10 (Catalog #AAA495398) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CXCL10 / IP-10, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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