Rabbit anti-Human CA9 Monoclonal Antibody | anti-CA9 antibody

CA9, CT (Carbonic Anhydrase 9, Carbonate Dehydratase IX, Carbonic Anhydrase IX, CA-IX, CAIX, Membrane Antigen MN, P54/58N, Renal Cell Carcinoma-associated Antigen G250, RCC-associated Antigen G250, pMW1, G250, MN)

Cat. Num. AAA649431

• Gene Names: CA9; MN; CAIX
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Supernatant; Supernatant
• Synonyms: CA9; Monoclonal Antibody; CT (Carbonic Anhydrase 9; Carbonate Dehydratase IX; Carbonic Anhydrase IX; CA-IX; CAIX; Membrane Antigen MN; P54/58N; Renal Cell Carcinoma-associated Antigen G250; RCC-associated Antigen G250; pMW1; G250; MN); Anti -CA9; anti-CA9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: D10C10
• Specificity: Recognizes endogenous level of total human CA9.
• Purity/Purification: Supernatant; Supernatant
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-CA9 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:1000; Immunohistochemistry (Paraffin): 1:100
• Immunogen: Synthetic peptide corresponding to residues surrounding Asp361 of human CA9.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of total extracts from LN18 and SW620 cells using CA9 Rabbit mAb.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded normal human colon using CA9 Rabbit mAb.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded cell pellets, LN18 (left) or HeLa (right), using CA9 Rabbit mAb.)

Related Product Information for anti-CA9 antibody

Carbonic anhydrases (CA) are a family of ancient zinc metalloenzymes found in almost all living organisms. All CAs can be divided into 3 distinct classes (a, b, and y) that evolved independently and have no significant homology in sequence and overall folding. All functional CAs catalyze the reversible hydration of CO2 into HCO3- and H+ and contain a zinc atom in the active sites essential for catalysis. There are many isoforms of CA in mammals and they all belong to the a class (1,2). CA9 is a member of alpha class, a plasma membrane protein with the catalytic domain in the extracellular space. Its expression is restricted to very few normal tissues (mainly the gastrointestinal tract) (2). CA9 expression is strongly induced by hypoxia and down-regulated by the wildtype von Hippel-Lindau (VHL) tumor suppressor protein. CA9 expression is increased in many types of tumor, especially in solid hypoxic tumors with a poor responsiveness to the conventional radio-and/or chemo-therapies; CA9 is considered as a tumor hypoxia marker and a promising target for cancer therapeutic intervention (3-5).

Product Categories/Family for anti-CA9 antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 169636420
• NCBI GeneID: 768
• NCBI Accession #: NP_001207.2
• NCBI GenBank Nucleotide #: NM_001216.2
• UniProt Accession #: Q16790; Q5T4R1
• Molecular Weight: 49,698 Da
• NCBI Official Full Name: carbonic anhydrase 9
• NCBI Official Synonym Full Names: carbonic anhydrase IX
• NCBI Official Symbol: CA9
• NCBI Official Synonym Symbols: MN; CAIX
• NCBI Protein Information: carbonic anhydrase 9; pMW1; CA-IX; P54/58N; membrane antigen MN; carbonic dehydratase; carbonate dehydratase IX; RCC-associated antigen G250; RCC-associated protein G250; renal cell carcinoma-associated antigen G250
• UniProt Protein Name: Carbonic anhydrase 9
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA9
• UniProt Synonym Gene Names: G250; MN; CA-IX; CAIX
• UniProt Entry Name: CAH9_HUMAN

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. Ref.12

Catalytic activity: H2CO3 = CO2 + H2O.

Cofactor: Zinc.

Enzyme regulation: Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA) and Foscarnet (phosphonoformate trisodium salt). Ref.10 Ref.11 Ref.14 Ref.15

Subunit structure: Forms oligomers linked by disulfide bonds. Ref.12 Ref.17

Subcellular location: Nucleus. Nucleus › nucleolus. Cell membrane; Single-pass type I membrane protein. Cell projection › microvillus membrane; Single-pass type I membrane protein. Note: Found on the surface microvilli and in the nucleus, particularly in nucleolus. Ref.8

Tissue specificity: Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.

Induction: By hypoxia. Ref.10 Ref.11 Ref.12 Ref.14 Ref.15

Post-translational modification: Asn-346 bears high-mannose type glycan structures.

Sequence similarities: Belongs to the alpha-carbonic anhydrase family.

Biophysicochemical propertiespH dependence:Optimum pH is 6.5. Ref.17