Western Blot (WB)
(Western Blot analysis of extracts from various cell types, using MBS601644)
Rabbit Rho Guanine Nucleotide Exchange Factor H1 Monoclonal Antibody | anti-ARHGEF2 antibody
Rho Guanine Nucleotide Exchange Factor H1 (ARHGEF-H1, GEF-H1)
Supernatant
Supernatant
Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Western Blot (WB)
(Western Blot analysis of extracts from various cell types, using MBS601644)
Western Blot (WB)
(Western Blot analysis of extracts from various cell types, using MBS601644)
Western Blot (WB)
(Immunoprecipitation of GEF-H1 from HeLa cell extracts, using MBS601644 Western blot was performed using the MBS601644.)
Western Blot (WB)
(Immunoprecipitation of GEF-H1 from HeLa cell extracts, using MBS601644 Western blot was performed using the MBS601644.)
NCBI and Uniprot Product Information
NCBI Description
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Jun 2009]
Uniprot Description
ARHGEF2: a microtubule-associated guanine nucleotide exchange factor for Rac and Rho GTPases. Mediates cross-talk between microtubules and the actin cytoskeleton. Contains a phorbol esters/diacylglycerol binding and PH domain.
Protein type: Motility/polarity/chemotaxis; GEFs; GEFs, Rac/Rho
Chromosomal Location of Human Ortholog: 1q21-q22
Cellular Component: Golgi apparatus; microtubule; tight junction; focal adhesion; protein complex; cytoplasmic membrane-bound vesicle; cytosol; cytoskeleton; cell soma; cytoplasm; spindle; dendritic shaft; vesicle
Molecular Function: protein binding; Rho guanyl-nucleotide exchange factor activity; Rho GTPase binding; zinc ion binding; microtubule binding; Rac guanyl-nucleotide exchange factor activity; Rac GTPase binding; transcription factor binding
Biological Process: mitosis; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; negative regulation of microtubule depolymerization; cell morphogenesis; positive regulation of interleukin-6 production; actin filament organization; positive regulation of tumor necrosis factor production; activation of NF-kappaB transcription factor; negative regulation of neurogenesis; regulation of cell proliferation; intracellular protein transport; positive regulation of peptidyl-tyrosine phosphorylation; regulation of small GTPase mediated signal transduction; establishment of mitotic spindle orientation; cell division; small GTPase mediated signal transduction; regulation of Rho protein signal transduction; innate immune response; positive regulation of transcription from RNA polymerase II promoter
Research Articles on ARHGEF2
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Product Notes
The ARHGEF2 arhgef2 (Catalog #AAA601644) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rho Guanine Nucleotide Exchange Factor H1 (ARHGEF-H1, GEF-H1) reacts with Hamster, Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Rho Guanine Nucleotide Exchange Factor H1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP). Suitable for use in ELISA, Western Blot and Immunoprecipitation. Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight. Immunoprecipitation: 1:50. Researchers should empirically determine the suitability of the ARHGEF2 arhgef2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rho Guanine Nucleotide Exchange Factor H1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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