Western Blot (WB)
(Western Blot analysis of extracts from COS-7 cells with MBS649420, untransfected or transfected with human SGK3, and untreated or treated with calf intestinal phosphatase (CIP), using Phospho-SGK3 (Thr320) Rabbit mAb (upper) or SGK3 (lower).)
Rabbit anti-Human SGK3 Monoclonal Antibody | anti-SGK3 antibody
SGK3, Phosphorylated (Thr320) (Serine/Threonine-protein Kinase Sgk3, Serum/Glucocorticoid-regulated Kinase 3, Serum/Glucocorticoid-regulated Kinase-like, SGK2, SGKL)
Supernatant
Supernatant
Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 1X TBS, 5% BSA, 0.1% Tween-20 at 4ºC with gentle shaking, overnight.
Western Blot (WB)
(Western Blot analysis of extracts from COS-7 cells with MBS649420, untransfected or transfected with human SGK3, and untreated or treated with calf intestinal phosphatase (CIP), using Phospho-SGK3 (Thr320) Rabbit mAb (upper) or SGK3 (lower).)
Western Blot (WB)
(Western Blot analysis of extracts from COS-7 cells with MBS649420, untransfected or transfected with human SGK3, and untreated or treated with calf intestinal phosphatase (CIP), using Phospho-SGK3 (Thr320) Rabbit mAb (upper) or SGK3 (lower).)
Western Blot (WB)
(Western Blot analysis of extracts from COS-7 cells, untransfected (-) or transfected with human SGK3 (+), using MBS649420, Phospho-SGK3 (Thr320) (upper) or SGK3 (lower).)
Western Blot (WB)
(Western Blot analysis of extracts from COS-7 cells, untransfected (-) or transfected with human SGK3 (+), using MBS649420, Phospho-SGK3 (Thr320) (upper) or SGK3 (lower).)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the Ser/Thr protein kinase family and encodes a phosphoprotein with a PX (phox homology) domain. The protein phosphorylates several target proteins and has a role in neutral amino acid transport and activation of potassium and chloride channels. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, proliferation, survival and migration. Up-regulates Na+ channels: SCNN1A/ENAC and SCN5A, K+ channels: KCNA3/KV1.3, KCNE1, KCNQ1 and KCNH2/HERG, epithelial Ca2+ channels: TRPV5 and TRPV6, chloride channel: BSND, creatine transporter: SLC6A8, Na+/dicarboxylate cotransporter: SLC13A2/NADC1, Na+-dependent phosphate cotransporter: SLC34A2/NAPI-2B, amino acid transporters: SLC1A5/ASCT2 and SLC6A19, glutamate transporters: SLC1A3/EAAT1, SLC1A6/EAAT4 and SLC1A7/EAAT5, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na+/H+ exchanger: SLC9A3/NHE3, and the Na+/K+ ATPase. Plays a role in the regulation of renal tubular phosphate transport and bone density. Phosphorylates NEDD4L and GSK3B. Positively regulates ER transcription activity through phosphorylation of FLII. Negatively regulates the function of ITCH/AIP4 via its phosphorylation and thereby prevents CXCR4 from being efficiently sorted to lysosomes. Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Ref.23 Ref.24 Ref.26 Ref.27 Ref.28 Ref.29
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation: Two specific sites, one in the kinase domain (Thr-320) and the other in the C-terminal regulatory region (Ser-486), need to be phosphorylated for its full activation.
Subunit structure: Interacts with GSK3B and FLII. Interacts with PDPK1 in a phosphorylation-dependent manner. Ref.7 Ref.17 Ref.24 Ref.27
Subcellular location: Cytoplasmic vesicle
By similarity. Early endosome. Recycling endosome. Note: Endosomal localization is a prerequisite for complete kinase activity. It is essential for its colocalization with the kinase responsible for phosphorylating Ser-486 thus allowing PDPK1 phosphorylation of Thr-320 resulting in complete activation of SGK3. Localized in vesicle-like structures and in the early endosome. Colocalizes with SLC9A3/NHE3 in the recycling endosomes. Ref.24 Ref.29
Tissue specificity: Expressed in most tissues with highest levels in pancreas, kidney liver, heart and brain and lower levels in lung, placenta and skeletal muscle. Expression is higher in ER-positive breast tumors than ER-negative breast tumors. Ref.30
Induction: Induced by estrogen/ER in breast cancer cells. Ref.30
Post-translational modification: Activated by phosphorylation on Ser-486 by an unknown kinase (may be mTORC2 but not confirmed), transforming it into a substrate for PDPK1 which then phosphorylates it on Thr-320.
Sequence similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.Contains 1 AGC-kinase C-terminal domain.Contains 1 protein kinase domain.Contains 1 PX (phox homology) domain.
Sequence caution: The sequence AAF12758.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Research Articles on SGK3
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Product Notes
The SGK3 sgk3 (Catalog #AAA649420) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SGK3, Phosphorylated (Thr320) (Serine/Threonine-protein Kinase Sgk3, Serum/Glucocorticoid-regulated Kinase 3, Serum/Glucocorticoid-regulated Kinase-like, SGK2, SGKL) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SGK3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 1X TBS, 5% BSA, 0.1% Tween-20 at 4ºC with gentle shaking, overnight. Researchers should empirically determine the suitability of the SGK3 sgk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SGK3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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