Human Nuclear matrix protein 22 (NMP-22) ELISA Kit | NMP-22 elisa kit

Human Nuclear matrix protein 22 (NMP-22) ELISA Kit

Cat. Num. AAA264227

• Reactivity: Human
• Synonyms: Nuclear matrix protein 22 (NMP-22); Human Nuclear matrix protein 22 (NMP-22) ELISA Kit; NMP-22 elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 2363

• Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant NMP-22 concentration.
• Assay Type: Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: Up to 0.06 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for NMP-22 elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human NMP-22 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 215274208
• NCBI GeneID: 7175
• Molecular Weight: 267,293 Da
• NCBI Official Full Name: Nucleoprotein TPR
• NCBI Official Synonym Full Names: translocated promoter region, nuclear basket protein
• NCBI Official Symbol: TPR
• NCBI Protein Information: nucleoprotein TPR; megator; tumor potentiating region; NPC-associated intranuclear protein; translocated promoter region protein; nuclear pore complex-associated protein TPR; translocated promoter region (to activated MET oncogene)
• UniProt Protein Name: Nucleoprotein TPR
• UniProt Gene Name: TPR
• UniProt Entry Name: TPR_HUMAN

NCBI Description

This gene encodes a large coiled-coil protein that forms intranuclear filaments attached to the inner surface of nuclear pore complexes (NPCs). The protein directly interacts with several components of the NPC. It is required for the nuclear export of mRNAs and some proteins. Oncogenic fusions of the 5' end of this gene with several different kinase genes occur in some neoplasias. [provided by RefSeq, Jul 2008]

Uniprot Description

TPR: a large coiled-coil nuclear pore protein that forms filamentous structures extending from nuclear pore complexes (NPCs) into the perinucleolar region. Together with Lamina-associated polypeptide 2 (LAP2) family members and Nup153, forms structural links between the nuclear lamina and the internal nuclear matrix. In interphase, localizes to the nucleoplasmic side of the nuclear pore complex (NPC) core structure, forming a fibrous structure called the nuclear basket. Essential for normal nucleocytoplasmic transport of proteins and mRNAs, plays a role in the establishment of nuclear-peripheral chromatin compartmentalization in interphase, and in the mitotic spindle checkpoint signaling during mitosis. Involved in the quality control and retention of unspliced mRNAs in the nucleus. Involved in the formation and/or maintenance of NPC-associated perinuclear heterochromatin exclusion zones (HEZs). Implicated in nuclear export of mRNAs transcribed from heat shock gene promoters; associates both with chromatin in the HSP70 promoter and with mRNAs transcribed from this promoter under stress-induced conditions. Translocations between human TPR and TrkA, MET, and RAF genes are oncogenic. Its N-terminus is involved in activation of oncogenic kinases.

Protein type: Membrane protein, peripheral; Nuclear envelope; Oncoprotein; Nucleoporin

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: kinetochore; nucleoplasm; nuclear membrane; extrinsic to membrane; cytoplasmic dynein complex; cytoplasm; nuclear envelope; nuclear pore; nucleus; nuclear inclusion body

Molecular Function: mRNA binding; tubulin binding; protein binding; protein homodimerization activity; protein anchor; transporter activity; heat shock protein binding; mitogen-activated protein kinase binding; chromatin binding; nucleocytoplasmic transporter activity

Biological Process: nuclear pore complex assembly; negative regulation of translational initiation; viral reproduction; positive regulation of heterochromatin formation; RNA export from nucleus; mitotic nuclear envelope disassembly; nuclear matrix organization and biogenesis; regulation of protein stability; pathogenesis; viral infectious cycle; negative regulation of transcription from RNA polymerase II promoter; glucose transport; RNA import into nucleus; regulation of protein export from nucleus; regulation of protein import into nucleus; positive regulation of protein export from nucleus; nuclear pore organization and biogenesis; protein import into nucleus; mitotic cell cycle spindle assembly checkpoint; viral transcription; protein export from nucleus; transmembrane transport; mitosis; cytokine and chemokine mediated signaling pathway; negative regulation of RNA export from nucleus; mRNA export from nucleus during heat stress; nuclear translocation of MAPK; positive regulation of protein import into nucleus; cell division; hexose transport; carbohydrate metabolic process; gene expression; mitotic cell cycle