Immunofluorescence (IF)
(Immunofluorescence staining of fixed K562 cells with anti-B7-H6 antibody 17B1.3 (MBS488753) Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 17B1.3 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Rabbit anti-Human B7-H6 Monoclonal Antibody | anti-B7-H6 antibody
Anti-B7-H6 [17B1.3]
Gene Names
NCR3LG1; B7H6; B7-H6; DKFZp686O24166
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence
Purity
Purified antibody. Protein A affinity purified
Synonyms
B7-H6; Monoclonal Antibody; Anti-B7-H6 [17B1.3]; B7H6; B7 homolog 6; Natural cytotoxicity triggering receptor 3 ligand 1; NCR3LG1; DKFZp686O2416; anti-B7-H6 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG, kappa
Clone Number
17B1.3
Specificity
mAb 17B1.3 specifically recognizes B7-H6. 17B1.3 reacted with human tumour cell lines constitutively expressing B7-H6 such as HeLa cells. Fab 17B1.3 does not interfere with the binding of B7-H6 to NKp30, but nevertheless blocks NK cell activation.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-B7-H6 antibody
Crystallization, Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
B7-H6 is not detected in normal human tissues at steady state but is expressed on tumour cells, so tumour cells can be detected by NK cells. This article describes the study of the mechanisms that govern the induction of B7-H6 on primary cells as well as its consequences in vitro and in vivo.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
PMID
23687088
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Immunofluorescence (IF)
(Immunofluorescence staining of fixed K562 cells with anti-B7-H6 antibody 17B1.3 (MBS488753) Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 17B1.3 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed K562 cells with anti-B7-H6 antibody 17B1.3 (MBS488753) Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 17B1.3 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-B7-H6 antibody
Recombinant monoclonal antibody to B7-H6. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 17B1.3.
Immunised mice with extracellular domain of human B7-H6 and selected on the basis of specific reactivity by P815.B7-H6, but not with P815.B7-H1.
Immunised mice with extracellular domain of human B7-H6 and selected on the basis of specific reactivity by P815.B7-H6, but not with P815.B7-H1.
Product Categories/Family for anti-B7-H6 antibody
References
Matta et al. Induction of B7-H6, a ligand for the natural killer cell-activating receptor NKp30, in inflammatory conditions Blood. 2013;122(3):394-404
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
80 kDa
NCBI Official Full Name
natural cytotoxicity triggering receptor 3 ligand 1
NCBI Official Synonym Full Names
natural killer cell cytotoxicity receptor 3 ligand 1
NCBI Official Symbol
NCR3LG1
NCBI Official Synonym Symbols
B7H6; B7-H6; DKFZp686O24166
NCBI Protein Information
natural cytotoxicity triggering receptor 3 ligand 1
UniProt Protein Name
Natural cytotoxicity triggering receptor 3 ligand 1
UniProt Gene Name
NCR3LG1
UniProt Synonym Gene Names
B7H6; B7-H6
NCBI Description
B7H6 belongs to the B7 family (see MIM 605402) and is selectively expressed on tumor cells. Interaction of B7H6 with NKp30 (NCR3; MIM 611550) results in natural killer (NK) cell activation and cytotoxicity (Brandt et al., 2009 [PubMed 19528259]).[supplied by OMIM, Jan 2011]
Uniprot Description
B7-H6: Triggers NCR3-dependent natural killer cell activation.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 11p15.1
Cellular Component: plasma membrane
Biological Process: regulation of immune response
Research Articles on B7-H6
Similar Products
Product Notes
The B7-H6 ncr3lg1 (Catalog #AAA488753) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-B7-H6 [17B1.3] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's B7-H6 can be used in a range of immunoassay formats including, but not limited to, Crystallization, Flow Cytometry (FC/FACS), Immunofluorescence (IF). B7-H6 is not detected in normal human tissues at steady state but is expressed on tumour cells, so tumour cells can be detected by NK cells. This article describes the study of the mechanisms that govern the induction of B7-H6 on primary cells as well as its consequences in vitro and in vivo. Researchers should empirically determine the suitability of the B7-H6 ncr3lg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B7-H6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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