B7-H6 / B7H6 active protein

Recombinant Human B7-H6 Protein (His Tag)(Active)

Cat. Num. AAA2545159

• Gene Names: NCR3LG1; B7H6; B7-H6; DKFZp686O24166
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Synonyms: B7-H6 / B7H6; Recombinant Human B7-H6 Protein (His Tag)(Active); Natural cytotoxicity triggering receptor 3 ligand 1; B7 homolog 6; B7-H6; NCR3LG1; B7H6; B7-H6 / B7H6 active protein

• Host: HEK293 Cells
• Purity/Purification: > 95 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4 5%Trehalose, 5% Mannitol, 0.01% Tween-80.
• Sequence: Met 1-Ser262

• Species: Human
• Tag: C-His
• Bio_activity: Measured by its binding ability in a functional ELISA. Immobilized human B7-H6-His at 10ug/mL (100uL/well) can bind human NCR3-Fch, the EC50 of human NCR3-Fch is 6-200ng/mL.
• Endotoxin: < 1.0 EU per ?g of the protein as determined by the LAL method.
• Reconstitution: We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex.; It is recommended that sterile water(800ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

SDS-PAGE

Related Product Information for B7-H6 / B7H6 active protein

Natural cytotoxicity triggering receptor 3 ligand 1(B7-H6) is a glycosylated member of the B7 family of immune costimulatory proteins. Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif. Both of the Ig-like domains carry N-linked glycosylation. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells. It does not show binding to NKp44, NKp46, or NKG2D. Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis. B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors.

NCBI and Uniprot Product Information

• NCBI GI #: 320461732
• NCBI GeneID: 374383
• NCBI Accession #: NP_001189368.1
• NCBI GenBank Nucleotide #: NM_001202439.2
• UniProt Accession #: Q68D85; Q7Z3M6
• Molecular Weight: 28.1 kDa
• NCBI Official Full Name: natural cytotoxicity triggering receptor 3 ligand 1
• NCBI Official Synonym Full Names: natural killer cell cytotoxicity receptor 3 ligand 1
• NCBI Official Symbol: NCR3LG1
• NCBI Official Synonym Symbols: B7H6; B7-H6; DKFZp686O24166
• NCBI Protein Information: natural cytotoxicity triggering receptor 3 ligand 1
• UniProt Protein Name: Natural cytotoxicity triggering receptor 3 ligand 1
• UniProt Gene Name: NCR3LG1
• UniProt Synonym Gene Names: B7H6; B7-H6

NCBI Description

B7H6 belongs to the B7 family (see MIM 605402) and is selectively expressed on tumor cells. Interaction of B7H6 with NKp30 (NCR3; MIM 611550) results in natural killer (NK) cell activation and cytotoxicity (Brandt et al., 2009 [PubMed 19528259]).[supplied by OMIM, Jan 2011]

Uniprot Description

B7-H6: Triggers NCR3-dependent natural killer cell activation.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: plasma membrane

Biological Process: regulation of immune response