Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood leukocytes with anti-CR1 antibody E11 (MBS488643) Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of D7C2 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Rabbit anti-Human CR1 Monoclonal Antibody | anti-CR1 antibody
Anti-CR1 [E11]
Reactivity
Human
Applications
ELISA, Immunofluorescence
Purity
Purified antibody. Protein A affinity purified
Synonyms
CR1; Monoclonal Antibody; Anti-CR1 [E11]; CD35; Type 1 complement receptor; C3b/C4b receptor; anti-CR1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG, kappa
Clone Number
E11
Specificity
E11 is reactive to neutrophilic granulocytes and eosinophils; monocytes; macrophages; lymphocytes; erythrocytes; dendritic reticulum cells; tonsil tissue section; spleen tissue section; lymph node tissue section; gut tissue section; kidney podocytes and kidney tissue sections; chronic lymphocytic leukemia; acute myelomonocytic leukemia; acute monocyte leukemia; B cell lines (JY, MST, BR17); EBV-infected B cell cultures; myeloid cell lines (U937, HL60, THP 1-0).
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CR1 antibody
ELISA (EIA), Western blot, Immunofluorescence (IF)
Application Notes
E11 fluorescence staining of normal blood cells was detectable on 90% of monocytes, 95% of neutrophils, and 14%-24% of lymphocytes (Hogg et al, 1984). Assay of E11 fluorescence-stained monocytes and neutrophils by EC3b rosette formation indicated that nearly all of the E11-stained cells formed rosettes with EC3b (Hogg et al, 1984). Treatment of erythrocytes, monocytes, neutrophils, and lymphocytes with E11 did not result in any detectable loss of CR1 activity as measured by EC3b rosette formation (Hogg et al, 1984). Immunofluorescence was used to determine E11 reactivity to a variety of cells, tissue sections, leukemias, and cell lines (Hogg et al, 1984). E11 has been used in ELISA experiments to quantify the amount of recombinant CR1 proteins (Park et al, 2013).
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
PMID
6368248
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood leukocytes with anti-CR1 antibody E11 (MBS488643) Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of D7C2 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood leukocytes with anti-CR1 antibody E11 (MBS488643) Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of D7C2 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-CR1 antibody
Recombinant monoclonal antibody to CR1. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma E11.
BALB/c mice were immunised with cells from acute monocytic leukemia patients.
BALB/c mice were immunised with cells from acute monocytic leukemia patients.
Product Categories/Family for anti-CR1 antibody
References
Hogg et al, 1984. Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1). Eur J Immunol. 1984 Mar;14(3):236-43.
Note on publication: Describes the original production of the antibody and its characterisation through immunofluorescence.
Note on publication: Describes the original production of the antibody and its characterisation through immunofluorescence.
NCBI and Uniprot Product Information
UniProt Accession #
Protein Family
Similar Products
Product Notes
The CR1 (Catalog #AAA488643) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CR1 [E11] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CR1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western blot, Immunofluorescence (IF). E11 fluorescence staining of normal blood cells was detectable on 90% of monocytes, 95% of neutrophils, and 14%-24% of lymphocytes (Hogg et al, 1984). Assay of E11 fluorescence-stained monocytes and neutrophils by EC3b rosette formation indicated that nearly all of the E11-stained cells formed rosettes with EC3b (Hogg et al, 1984). Treatment of erythrocytes, monocytes, neutrophils, and lymphocytes with E11 did not result in any detectable loss of CR1 activity as measured by EC3b rosette formation (Hogg et al, 1984). Immunofluorescence was used to determine E11 reactivity to a variety of cells, tissue sections, leukemias, and cell lines (Hogg et al, 1984). E11 has been used in ELISA experiments to quantify the amount of recombinant CR1 proteins (Park et al, 2013). Researchers should empirically determine the suitability of the CR1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CR1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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