Mouse anti-Human ATD3A Monoclonal Antibody | anti-ATD3A antibody

ATD3A (384) Mouse mAb

Cat. Num. AAA435098

• Gene Names: ATAD3A; HAYOS
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
• Synonyms: ATD3A; Monoclonal Antibody; ATD3A (384) Mouse mAb; ATD3A(384) Mouse mAb; anti-ATD3A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 586

• Applicable Applications for anti-ATD3A antibody: Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000-1:5000; IP: 10 tests; FC/FACS: 1:100-1:200
• Source Note: This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human ATD3A
• Preparation and Storage: Store at -20 degree C. Stable for one year from the date of shipment.

Western Blot (WB)

Immunoprecipitation (IP)

(ATD3A(384) was immunopre-cipitated from 1mg of Jurkat cells membrane fraction, blotted with M25064 of 10 ug. Western blot was performed from the immunopre-cipitate using M25064 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Predicted band size : 66 KDaObserved band size : 66 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling ATD3A(384) with M25064 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (blue) and an unlabelled control (cells without incubation with primary antibody, green). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)

Related Product Information for anti-ATD3A antibody

ATAD3A and ATAD3B (MIM 612317) are mitochondrial membrane proteins that contribute to the stabilization of large mitochondrial DNA (mtDNA)-protein complexes called nucleoids.

Product Categories/Family for anti-ATD3A antibody

Cell Marker; Subcellular Marker; Membrane

NCBI and Uniprot Product Information

• NCBI GI #: 283436222
• NCBI GeneID: 55210
• NCBI Accession #: NP_001164006.1
• NCBI GenBank Nucleotide #: NM_001170535.2
• UniProt Accession #: Q9NVI7; Q5SV23; Q8N275; Q96A50; B3KPB3; D2K8Q1; G3V1I6
• Molecular Weight: Predicted MW: 66kDa
• NCBI Official Full Name: ATPase family AAA domain-containing protein 3A isoform 2
• NCBI Official Synonym Full Names: ATPase family, AAA domain containing 3A
• NCBI Official Symbol: ATAD3A
• NCBI Official Synonym Symbols: HAYOS
• NCBI Protein Information: ATPase family AAA domain-containing protein 3A
• UniProt Protein Name: ATPase family AAA domain-containing protein 3A
• UniProt Gene Name: ATAD3A

NCBI Description

This gene encodes a ubiquitously expressed mitochondrial membrane protein that contributes to mitochondrial dynamics, nucleoid organization, protein translation, cell growth, and cholesterol metabolism. This gene is a member of the ATPase family AAA-domain containing 3 gene family which, in humans, includes two other paralogs. Naturally occurring mutations in this gene are associated with distinct neurological syndromes including Harel-Yoon syndrome. High-level expression of this gene is associated with poor survival in breast cancer patients. A homozygous knockout of the orthologous gene in mice results in embryonic lethality at day 7.5 due to growth retardation and defective development of the trophoblast lineage. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2017]

Uniprot Description

Essential for mitochondrial network organization, mitochondrial metabolism and cell growth at organism and cellular level. May play an important role in mitochondrial protein synthesis. May also participate in mitochondrial DNA replication. May bind to mitochondrial DNA D-loops and contribute to nucleoid stability. Required for enhanced channeling of cholesterol for hormone-dependent steroidogenesis.