Western Blot (WB)
(Western Blot; Sample: Porcine Cerebrum lysate; Primary Ab: 2ug/ml Mouse Anti-Human AST Antibody)
Mouse anti-Human Aspartate Aminotransferase (AST) Monoclonal Antibody | anti-AST antibody
Monoclonal Antibody to Aspartate Aminotransferase (AST)
Immunohistochemistry: 5-30ug/mL
Immunocytochemistry: 5-30ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot; Sample: Porcine Cerebrum lysate; Primary Ab: 2ug/ml Mouse Anti-Human AST Antibody)
Western Blot (WB)
(Western Blot; Sample: Porcine Cerebrum lysate; Primary Ab: 2ug/ml Mouse Anti-Human AST Antibody)
Western Blot (WB)
(Sample: Human Liver lysate;Primary Ab: 3ug/ml Mouse Anti-Human AST AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: )
Western Blot (WB)
(Sample: Human Liver lysate;Primary Ab: 3ug/ml Mouse Anti-Human AST AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: )
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Liver Tissue;Primary Ab: 30ug/ml Mouse Anti-Human AST AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: )
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Liver Tissue;Primary Ab: 30ug/ml Mouse Anti-Human AST AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: )
NCBI and Uniprot Product Information
NCBI Description
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]
Uniprot Description
GOT1: Plays a key role in amino acid metabolism. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Protein type: Amino Acid Metabolism - cysteine and methionine; Transferase; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - tyrosine; EC 2.6.1.1; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.3
Chromosomal Location of Human Ortholog: 10q24.1-q25.1
Cellular Component: lysosome; cytoplasm; nerve terminal; nucleus; cytosol
Molecular Function: cysteine transaminase activity; phosphatidylserine decarboxylase activity; carboxylic acid binding; aspartate transaminase activity; pyridoxal phosphate binding
Biological Process: glutamate metabolic process; oxaloacetate metabolic process; glutamate catabolic process to aspartate; response to glucocorticoid stimulus; glucose metabolic process; aspartate biosynthetic process; pathogenesis; gluconeogenesis; 2-oxoglutarate metabolic process; methionine salvage; cellular response to insulin stimulus; sulfur amino acid metabolic process; glycerol biosynthetic process; fatty acid homeostasis; carbohydrate metabolic process; aspartate catabolic process; aspartate metabolic process; amino acid biosynthetic process; glutamate catabolic process to 2-oxoglutarate; polyamine metabolic process
Disease: Aspartate Aminotransferase, Serum Level Of, Quantitative Trait Locus 1
Research Articles on AST
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Product Notes
The AST got1 (Catalog #AAA2111221) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Aspartate Aminotransferase (AST) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Aspartate Aminotransferase (AST) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western Blotting: 0.5-3ug/mL Immunohistochemistry: 5-30ug/mL Immunocytochemistry: 5-30ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the AST got1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aspartate Aminotransferase (AST), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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