Frataxin Recombinant Protein | FXN recombinant protein

Recombinant Frataxin (FXN)

Cat. Num. AAA2030063

• Gene Names: FH; ATFH; F4C21.17; F4C21_17; FH; frataxin homolog
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Frataxin; Recombinant Frataxin (FXN); FXN recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Concentration: 6.4 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LHVTVNAGAT RHAHLNLHYL QILNIKKQSV CVVHLRNLGT LDNPSSLDET AYERLAEETL DSLAEFFEDL ADKPYTLEDY DVSFGDGVLT IKLGGDLGTY VINKQTPNKQ IWLSSPSSGP KRYDWTGKNW VYSHDGVSLHELLARELTKA LNTKLDLSSL AYSGKGT
• Sequence Length: 187

• Applicable Applications for FXN recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 22.4kDa
• Accurate Molecular Mass (KD): 24kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Leu41~Thr207
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 42566266
• NCBI GeneID: 828013
• NCBI Accession #: NP_192233.2
• NCBI GenBank Nucleotide #: NM_116562.3
• Molecular Weight: 21,430 Da
• NCBI Official Full Name: frataxin
• NCBI Official Symbol: FH
• NCBI Official Synonym Symbols: ATFH; F4C21.17; F4C21_17; FH; frataxin homolog
• NCBI Protein Information: frataxin
• UniProt Protein Name: Frataxin, mitochondrial
• Protein Family: Frataxin
• UniProt Gene Name: FH
• UniProt Synonym Gene Names: Fxn
• UniProt Entry Name: FRDA_ARATH

NCBI Description

Encodes AtFH, a frataxin homolog. Frataxin is required for the biogenesis of mitochondria in different organisms. AtFH knock-out mutants are lethal. Required for full activity of mitochondrial Fe-S proteins. Deficiency of AtFH induces oxidative stress.

Uniprot Description

Promotes the biosynthesis of heme as well as the assembly and repair of iron-sulfur clusters by delivering Fe2+ to proteins involved in these pathways (PubMed:17092311). May play a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe2+ to Fe3+ (PubMed:17092311). May be able to store large amounts of the metal in the form of a ferrihydrite mineral by oligomerization (PubMed:17092311). Binds to the mitochondrial cysteine desulfurase NIFS1 and increases its activity (PubMed:22511606).