Western Blot (WB)
(Sample: Rat Spleen lysate Primary Ab: 2ug/mL Mouse Anti-Human NGF-2 Antibody Second Ab)
Mouse anti-Human Nerve Growth Factor (NGF) Monoclonal Antibody | anti-NGF antibody
Monoclonal Antibody to Nerve Growth Factor (NGF)
Immunohistochemistry: 5-50ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 24 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Sample: Rat Spleen lysate Primary Ab: 2ug/mL Mouse Anti-Human NGF-2 Antibody Second Ab)
Western Blot (WB)
(Sample: Rat Spleen lysate Primary Ab: 2ug/mL Mouse Anti-Human NGF-2 Antibody Second Ab)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Cerebrum Tissue; Primary Ab: 40ug/ml Mouse Anti-Human NGF Antibody Second Ab: 2ug/mL HRPLinked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: )
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Cerebrum Tissue; Primary Ab: 40ug/ml Mouse Anti-Human NGF Antibody Second Ab: 2ug/mL HRPLinked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: )
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008]
Uniprot Description
NGF: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Homodimer. Belongs to the NGF-beta family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1p13.1
Cellular Component: extracellular space; Golgi lumen; cytoplasmic membrane-bound vesicle; extracellular region; endosome
Molecular Function: metalloendopeptidase inhibitor activity; protein binding; nerve growth factor receptor binding; growth factor activity; receptor signaling protein activity
Biological Process: response to nicotine; circadian rhythm; response to peptide hormone stimulus; nerve growth factor receptor signaling pathway; activation of MAPKK activity; positive regulation of apoptosis; adult locomotory behavior; regulation of neuron differentiation; positive regulation of axon extension; response to glucocorticoid stimulus; regulation of neurotransmitter secretion; response to lipopolysaccharide; regulation of caspase activity; sensory perception of pain; regulation of axonogenesis; negative regulation of cell cycle; nerve growth factor processing; response to radiation; cell-cell signaling; small GTPase mediated signal transduction; negative regulation of neuron apoptosis; response to electrical stimulus; inflammatory response; response to drug; positive regulation of neuron maturation; phosphoinositide-mediated signaling; positive regulation of protein amino acid autophosphorylation; positive regulation of nerve growth factor receptor signaling pathway; regulation of release of sequestered calcium ion into cytosol; memory; peripheral nervous system development; neuron apoptosis; induction of apoptosis via death domain receptors; response to mechanical stimulus; phospholipase C activation; response to ozone; Ras protein signal transduction; positive regulation of axonogenesis; positive regulation of transcription factor activity; neurite morphogenesis; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis
Disease: Neuropathy, Hereditary Sensory And Autonomic, Type V
Research Articles on NGF
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Product Notes
The NGF ngf (Catalog #AAA2111093) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Nerve Growth Factor (NGF) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nerve Growth Factor (NGF) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-50ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the NGF ngf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nerve Growth Factor (NGF), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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