Rabbit OTUD5 Polyclonal Antibody | anti-OTUD5 antibody

OTUD5 Antibody

Cat. Num. AAA150538

• Gene Names: OTUD5; DUBA
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: OTUD5 Antibody is affinity chromatography purified via peptide column.
• Synonyms: OTUD5; Polyclonal Antibody; OTUD5 Antibody; DUBA; OTU domain-containing protein 5; Deubiquitinating enzyme A; OTU domain containing 5; anti-OTUD5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: OTUD5 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 641

• Applicable Applications for anti-OTUD5 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: OTUD5 antibody can be used for detection of OTUD5 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: OTUD5 antibody was raised against a 13 amino acid synthetic peptide near the carboxy terminus of the human OTUD5.
• Buffer: OTUD5 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: OTUD5 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of OTUD5 in human kidney lysate with OTUD5 antibody at (A) 1 and (B) 2 μg/mL. )

Immunohistochemistry (IHC)

(Immunohistochemistry of OTUD5 in mouse kidney tissue with OTUD5 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of OTUD5 in Human Kidney cells with OTUD5 antibody at 20 μg/mL.)

Related Product Information for anti-OTUD5 antibody

OTUD5 Antibody: OTUD5 is a member of the OTU (ovarian tumor) domain-containing cysteine protease superfamily. The OTU domain confers deubiquitinase activity and OTUD5 has been shown to suppress the type I interferon (IFN-I)-dependent innate immune response by cleaving the polyubiquitin chain from TRAF3, an essential type I interferon adaptor protein. Cleavage results in disassociation of TRAF3 from a downstream signaling complex containing TBK1 and the disruption of the IFN-I signaling cascade, indicating that OTUD5 acts as a negative regulator of innate immune responses. It has been suggested that by suppressing IFN-I production, OTUD5 may function to inhibit the emergence of certain autoimmune disorders such as systemic lupus erythematosus. Multiple isoforms of OTUD5 are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 119571108
• NCBI GeneID: 55593
• UniProt Accession #: Q96G74; Q4KMN9; Q8N6T5; Q9H650; Q9H9U0; Q9NT65; B4DGG7; G5E9D7
• Molecular Weight: 60,210 Da
• NCBI Official Full Name: OTU domain containing 5, isoform CRA_b
• NCBI Official Synonym Full Names: OTU deubiquitinase 5
• NCBI Official Symbol: OTUD5
• NCBI Official Synonym Symbols: DUBA
• NCBI Protein Information: OTU domain-containing protein 5; OTU domain containing 5; deubiquinating enzyme A; deubiquitinating enzyme A
• UniProt Protein Name: OTU domain-containing protein 5
• Protein Family: OTU domain-containing protein
• UniProt Gene Name: OTUD5
• UniProt Synonym Gene Names: DUBA
• UniProt Entry Name: OTUD5_HUMAN

NCBI Description

This gene encodes a member of the OTU (ovarian tumor) domain-containing cysteine protease superfamily. The OTU domain confers deubiquitinase activity and the encoded protein has been shown to suppress the type I interferon-dependent innate immune response by cleaving the polyubiquitin chain from an essential type I interferon adaptor protein. Cleavage results in disassociation of the adaptor protein from a downstream signaling complex and disruption of the type I interferon signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Oct 2008]

Uniprot Description

OTUD5: a protein of unknown function. Contains a OTU-like cysteine protease domain.

Protein type: EC 3.4.19.12; Protease; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: cytosol

Molecular Function: ubiquitin-specific protease activity

Biological Process: innate immune response; response to lipopolysaccharide; proteolysis; negative regulation of interferon type I production