Rabbit anti-Mouse Myosin IE (MYO1E) Polyclonal Antibody | anti-MYO1E antibody

Polyclonal Antibody to Myosin IE (MYO1E)

Cat. Num. AAA2004711

• Gene Names: Myo1e; myr 3; AA407778; myosin-1e; 2310020N23Rik; 9130023P14Rik
• Reactivity: Mouse
• Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Myosin IE (MYO1E); Polyclonal Antibody; Polyclonal Antibody to Myosin IE (MYO1E); anti-MYO1E antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Concentration: 0.23mg/ml (varies by lot)

• Applicable Applications for anti-MYO1E antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Western blotting: 0.5-2ug/mL; Immunohistochemistry: 5-20ug/mL; Immunocytochemistry: 5-20ug/mL; Optimal working dilutions must be determined by end user.
• Organism Species: Mus musculus (Mouse)
• Source: Polyclonal antibody preparation
• Cross Reactivity: Human, Mouse
• Traits: Liquid
• Immunogen: Recombinant MYO1E (Lys772~Ser1033 (Accession # E9Q634)) expressed in E.coli ( MBS2010151).
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 4 degree C for frequent use.; Aliquot and store at -20 degree C for 24 months.; ; Stability Test:; thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot; Sample: Recombinant MYO1E, Mouse.)

Western Blot (WB)

(Western Blot; Sample: Mouse Liver lysate;Primary Ab: 1ug/ml Rabbit Anti-Mouse MYO1E AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: )

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Mouse Kidney Tissue;Primary Ab: 20ug/ml Rabbit Anti-Mouse MYO1E AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: )

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples:Mouse Kidney Tissue; Primary Ab: 20ug/ml Rabbit Anti-Mouse MYO1E AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG PolyclonalAntibody (Catalog: )

NCBI and Uniprot Product Information

• NCBI GI #: 68299824
• NCBI GeneID: 71602
• NCBI Accession #: NP_851417.2
• NCBI GenBank Nucleotide #: NM_181072.3
• UniProt Accession #: E9Q634; Q80X36; Q91ZI4
• NCBI Official Full Name: unconventional myosin-Ie
• NCBI Official Synonym Full Names: myosin IE
• NCBI Official Symbol: Myo1e
• NCBI Official Synonym Symbols: myr 3; AA407778; myosin-1e; 2310020N23Rik; 9130023P14Rik
• NCBI Protein Information: unconventional myosin-Ie
• UniProt Protein Name: Unconventional myosin-Ie
• Protein Family: Unconventional myosin
• UniProt Gene Name: Myo1e
• UniProt Synonym Gene Names: Myr3

Uniprot Description

MYO1E: Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. Binds to membranes containing anionic phospholipids via its tail domain. Required for normal morphology of the glomerular basement membrane, normal development of foot processes by kidney podocytes and normal kidney function. In dendritic cells, may control the movement of class II-containing cytoplasmic vesicles along the actin cytoskeleton by connecting them with the actin network via ARL14EP and ARL14. Defects in MYO1E are the cause of focal segmental glomerulosclerosis type 6 (FSGS6). A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and progressive decline in renal function. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. FSGS6 is a childhood-onset disorder resulting in nephrotic syndrome, which includes massive proteinuria, hypoalbuminemia, hyperlipidemia, and edema.

Protein type: Actin-binding; Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 9 D|9 39.41 cM

Cellular Component: adherens junction; brush border; cell junction; clathrin-coated endocytic vesicle; cytoplasm; cytoplasmic vesicle; cytoskeleton; extracellular exosome; intercellular junction; myosin complex; protein complex

Molecular Function: actin binding; actin filament binding; ATP binding; ATPase activity, coupled; calmodulin binding; lipid binding; motor activity; nucleotide binding; phosphatidylinositol binding; protein complex binding

Biological Process: endocytosis; glomerular basement membrane development; glomerular filtration; glomerular visceral epithelial cell development; hemopoiesis; in utero embryonic development; kidney development; nitrogen compound metabolic process; platelet-derived growth factor receptor signaling pathway; post-embryonic hemopoiesis; vasculogenesis