Mouse Androgen Receptor Monoclonal Antibody

Androgen Receptor

Cat. Num. AAA370385

• Reactivity: Species Reactivity: Human; Reactivity: Paraffin, Frozen
• Synonyms: Androgen Receptor; Monoclonal Antibody; anti-Androgen Receptor antibody

• Host: Mouse
• Reactivity: Species Reactivity: Human; Reactivity: Paraffin, Frozen
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: BSB-4

• Immunogen: A synthetic peptide corresponding to residues in the N-terminus of human Androgen Receptor protein.
• Localization: Nuclear
• Control: Prostate, Prostatic Adenocarcinoma
• Precautions: 1. For professional users only. Results should be interpreted by a qualified medical professional.; 2. This product contains <0.1% sodium azide (NaN3) as a preservative.; Ensure proper handling procedures are used with this reagent.; 3. Always wear personal protective equipment such as laboratory coat, goggles and gloves when handling reagents.; 4. Dispose of unused solution with copious amount of water.; 5. Do not ingest reagent. If reagent is ingested, seek medical advice immediately.; 6. Avoid contact with eyes. If contact occurs, flush with large quantities of water.; 7. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).; 8. For additional safety information refer to Safety Data Sheet for this product.; 9. For complete recommendations for handling biological specimens, please refer to the CDC document, “Guidelines for Safe Work Practices in Human and AMDL” (see References in this document).
• Specimen Preparation: Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation for best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate ImmunoDNA Retriever with EDTA or ImmunoDNA Digestor. See reverse side for complete protocol. Tissue should remain hydrated via use of Immuno/DNA Washer solutions.; Frozen sections and cell preparations: The antibody can be used on acetone-fixed frozen sections and acetone-fixed cell preparations.
• IHC Protocol: 1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positively charged slides such as Bio SB Hydrophilic Plus Slides.; 2. Air dry for 2 hours at 58° C.; 3. Deparaffinize, dehydrate and rehydrate tissues.; 4. Subject tissues to heat induced epitope retrieval (HIER) using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA.; 5. Any of three heating methods may be used:; ; a. TintoRetriever Pressure Cooker or Equivalent Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place on trivet in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.; b. TintoRetriever PT Module or Water Bath Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.; c. Conventional Steamer Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a steamer, cover and steam for 30-60 minutes.; ; 6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.; 7. For manual IHC, perform antibody incubation at ambient temperature. For automated IHC methods, perform antibody incubation according to instrument manufacturer’s instructions.; 8. Wash slides with ImmunoDNA washer or DI water.; 9. Continue IHC protocol. Wash slides between each step with ImmunoDNA washer solution.
• Mounting Protocols: For detailed instructions using biodegradable permanent mounting media such as XyGreen PermaMounter or organic solvent based resin such as PermaMounter please contact Technical Support.
• Stability: This product is stable up to the expiration date on the poduct label. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions.
• Mounting Protocols: For detailed instructions using biodegradable permanent mounting media such as XyGreen PermaMounter or organic solvent based resin such as PermaMounter, refer to technical support.
• Preparation and Storage: Store at 2-8°C (Control Slides: Store at 20-25°C)

Related Product Information for anti-Androgen Receptor antibody

The androgen receptor (AR) is a type of nuclear receptor which is activated by binding of either of the androgenic hormones testosterone or dihydrotestosterone. The main function of the androgen receptor is as a DNA-binding transcription factor which regulates gene expression. However, the androgen receptor has additional functions independent of DNA binding. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and the brain.

This antibody reacts with the androgen receptor and also with the newly-described A form of the receptor. This antibody does not cross-react with estrogen, progesterone or glucocorticoid receptors. Abnormalities in the AR-signaling pathway have been linked to a number of diseases, including Prostate Cancer, Kennedy’s Disease and male infertility.

Product Categories/Family for anti-Androgen Receptor antibody

Predilute Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4262811
• NCBI Official Full Name: androgen receptor, partial
• Protein Family: Androgen receptor