Rabbit ICSBP / IRF8 Polyclonal Antibody | anti-ICSBP antibody

Rabbit Polyclonal to Human ICSBP / IRF8

Cat. Num. AAA240342

• Gene Names: IRF8; ICSBP; IRF-8; ICSBP1; IMD32A; IMD32B; H-ICSBP
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: ICSBP / IRF8; Polyclonal Antibody; Rabbit Polyclonal to Human ICSBP / IRF8; Anti-ICSBP / IRF8 Antibody (C-Terminus) IHC-plus; IRF8; H-ICSBP; Interferon regulatory factor 8; ICSBP; ICSBP1; IRF-8; Human ICSBP; anti-ICSBP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: peptide corresponding to 16 amino acids near the carboxy-terminus of human IRF8
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 426

• Applicable Applications for anti-ICSBP antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: Immunohistochemistry: MBS240342 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.; The optimal working concentration for MBS240342 was determined to be 5 ug/ml.
• Immunogen Species: ICSBP/IRF8 antibody was raised against Human
• Immunogen Type: Synthetic peptide
• Antigen Modification: C-Terminus
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-IRF8 antibody IHC of human small intestine, Peyer's patch. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of IRF8 in K562 cells with IRF8 antibody at 10 ug/ml.)

Western Blot (WB)

(Western blot of IRF8 in human thymus tissue lysate with IRF8 antibody at 1ug/ml in (A) the presence and (B) absence of blocking peptide.)

Related Product Information for anti-ICSBP antibody

Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta.

NCBI and Uniprot Product Information

• NCBI GI #: 4504567
• NCBI GeneID: 3394
• NCBI Accession #: NP_002154.1
• NCBI GenBank Nucleotide #: NM_002163.2
• UniProt Accession #: Q02556; A0AV82
• Molecular Weight: 48,356 Da
• NCBI Official Full Name: interferon regulatory factor 8
• NCBI Official Synonym Full Names: interferon regulatory factor 8
• NCBI Official Symbol: IRF8
• NCBI Official Synonym Symbols: ICSBP; IRF-8; ICSBP1; IMD32A; IMD32B; H-ICSBP
• NCBI Protein Information: interferon regulatory factor 8; interferon consensus sequence binding protein 1
• UniProt Protein Name: Interferon regulatory factor 8
• UniProt Gene Name: IRF8
• UniProt Synonym Gene Names: ICSBP1; IRF-8; H-ICSBP; ICSBP
• UniProt Entry Name: IRF8_HUMAN

NCBI Description

Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection. [provided by RefSeq, Jul 2008]

Uniprot Description

IRF8: Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system. Interacts (via C-terminus) with TRIM21 (via C-terminus). Interacts with COPS2. By IFNG/IFN-gamma. Predominantly expressed in lymphoid tissues. Belongs to the IRF family.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 16q24.1

Cellular Component: nucleoplasm; cytosol

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding

Biological Process: transcription from RNA polymerase II promoter; positive regulation of interferon-gamma production; positive regulation of interleukin-12 production; cytokine and chemokine mediated signaling pathway; defense response to bacterium; immune response; myeloid cell differentiation; negative regulation of transcription from RNA polymerase II promoter; defense response to protozoan; phagocytosis

Disease: Monocyte And Dendritic Cell Deficiency, Autosomal Recessive; Immunodeficiency 32a