anti-Human TREM2 / TREM-2 Polyclonal Antibody | anti-TREM2 antibody

Goat Polyclonal to Human TREM2 / TREM-2

Cat. Num. AAA242024

• Gene Names: TREM2; TREM-2; Trem2a; Trem2b; Trem2c
• Reactivity: Human
• Applications: Immunohistochemistry, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: TREM2 / TREM-2; Polyclonal Antibody; Goat Polyclonal to Human TREM2 / TREM-2; Anti-TREM2 / TREM-2 Antibody (C-Terminus) IHC-plus; TREM2; Trem2b; TREM-2; Trem2a; Trem2c; Human TREM2; anti-TREM2 antibody

• Host: TREM2/TREM-2 antibody was produced in Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human TREM2
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
• Concentration: 0.5mg/ml (varies by lot)
• Sequence Length: 199-212

• Applicable Applications for anti-TREM2 antibody: Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Peptide Enzyme-Linked Immunosorbent Assay
• Application Notes: IHC - Paraffin (2.5 µg/ml); Peptide Enzyme-Linked Immunosorbent Assay (1:128000); ; Usage:; Immunohistochemistry: MBS242024 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS242024 was determined to be 2.5 ug/ml.; (Optimal dilution to be determined by the researcher.)
• Target: TREM2/TREM-2
• Immunogen Species: TREM2/TREM-2 antibody was raised against Human
• Immunogen: TREM2/TREM-2 antibody was raised against peptide with sequence C-HGQKPGTHPPSELD, from the C-Terminus of protein sequence according to NP_061838.1.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-TREM2 antibody IHC of human spleen, macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 9507203
• NCBI GeneID: 54209
• NCBI Accession #: NP_061838.1
• NCBI GenBank Nucleotide #: NM_018965.3
• UniProt Accession #: Q9NZC2; Q8N5H8; Q8WYN6
• NCBI Official Full Name: triggering receptor expressed on myeloid cells 2 isoform 1
• NCBI Official Synonym Full Names: triggering receptor expressed on myeloid cells 2
• NCBI Official Symbol: TREM2
• NCBI Official Synonym Symbols: TREM-2; Trem2a; Trem2b; Trem2c
• NCBI Protein Information: triggering receptor expressed on myeloid cells 2; triggering receptor expressed on monocytes 2; triggering receptor expressed on myeloid cells 2a
• UniProt Protein Name: Triggering receptor expressed on myeloid cells 2
• Protein Family: Triggering receptor expressed on myeloid cells
• UniProt Gene Name: TREM2
• UniProt Synonym Gene Names: TREM-2
• UniProt Entry Name: TREM2_HUMAN

NCBI Description

This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012]

Uniprot Description

TREM2: May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL); also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: intracellular membrane-bound organelle; extracellular region; plasma membrane; integral to membrane

Molecular Function: peptidoglycan binding; lipopolysaccharide binding; receptor activity

Biological Process: axon guidance; positive regulation of peptidyl-tyrosine phosphorylation; detection of lipopolysaccharide; detection of peptidoglycan; innate immune response; positive regulation of calcium-mediated signaling; humoral immune response; positive regulation of antigen processing and presentation of peptide antigen via MHC class II

Disease: Polycystic Lipomembranous Osteodysplasia With Sclerosing Leukoencephalopathy