Western Blot (WB)
(Histone H3K9me0 mAb tested by Western blot. Histone H3K9me0 detection by Western blot. The analysis was performed using HeLa acid extract.)
Mouse Histone H3K9me0 Monoclonal Antibody | anti-H3K9me0 antibody
Histone H3K9me0 antibody (mAb)(Clone 9B1-2G6)
Reactivity
Human, Wide Range Predicted
Applications
Western Blot
Purity
Protein A Chromatography
Synonyms
Histone H3K9me0; Monoclonal Antibody; Histone H3K9me0 antibody (mAb)(Clone 9B1-2G6); anti-H3K9me0 antibody
Host
Mouse
Reactivity
Human, Wide Range Predicted
Clonality
Monoclonal
Isotype
IgG1
Clone Number
9B1-2G6
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-H3K9me0 antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 0.5-2ug/ml dilution
WB: 0.5-2ug/ml dilution
Conjugation
Methylated
Immunogen
This antibody was raised against a synthetic branched peptide corresponding to amino acids surrounding Lys9 of human Histone H3.
Abs Residue
Lys
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(Histone H3K9me0 mAb tested by Western blot. Histone H3K9me0 detection by Western blot. The analysis was performed using HeLa acid extract.)
Western Blot (WB)
(Histone H3K9me0 mAb tested by Western blot. Histone H3K9me0 detection by Western blot. The analysis was performed using HeLa acid extract.)
Dot Blot (DB)
(Histone H3K9me0 mAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3K9me0 mAb for detection of unmethylated Lys9 of Histone H3. 50 pmol of recombinant Histone H3 proteins containing various modifications were spotted onto PVDF and probed with the antibody at 1 ug/mL. Lane 1: Histone H3 unmodified. Lane 2: Histone H3K4me3. Lane 3: Histone H3K9me3. Lane 4: Histone H3 K9ac. Lane 5: Histone H3 K14ac. Lane 6: Histone H3K4ac. Lane 7: Histone H3 S10ph.)
Dot Blot (DB)
(Histone H3K9me0 mAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3K9me0 mAb for detection of unmethylated Lys9 of Histone H3. 50 pmol of recombinant Histone H3 proteins containing various modifications were spotted onto PVDF and probed with the antibody at 1 ug/mL. Lane 1: Histone H3 unmodified. Lane 2: Histone H3K4me3. Lane 3: Histone H3K9me3. Lane 4: Histone H3 K9ac. Lane 5: Histone H3 K14ac. Lane 6: Histone H3K4ac. Lane 7: Histone H3 S10ph.)
Related Product Information for anti-H3K9me0 antibody
Short Description: Our Histone H3K9me0 antibody (mAb)(Clone 9B1-2G6) was raised in a Mouse host. It has been validated for use in Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Methylation of lysines occur as four states: unmethylated (me0), monomethyl (me1), dimethyl (me2) and trimethyl (me3). The me0 state of lysine is recognized as biologically relevant and a number of proteins containing PhD fingers, ADD and WD40 domains are known to associate with unmodified lysines.
Background: Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Methylation of lysines occur as four states: unmethylated (me0), monomethyl (me1), dimethyl (me2) and trimethyl (me3). The me0 state of lysine is recognized as biologically relevant and a number of proteins containing PhD fingers, ADD and WD40 domains are known to associate with unmodified lysines.
Product Categories/Family for anti-H3K9me0 antibody
NCBI and Uniprot Product Information
Molecular Weight
17kDa
Similar Products
Product Notes
The H3K9me0 (Catalog #AAA388879) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Histone H3K9me0 antibody (mAb)(Clone 9B1-2G6) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3K9me0 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 0.5-2ug/ml dilution. Researchers should empirically determine the suitability of the H3K9me0 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3K9me0, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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