Rabbit anti-Human 2B4/CD244 Monoclonal Antibody | anti-CD244 antibody

Recombinant Anti-2B4/CD244 Antibody, Rabbit Monoclonal

Cat. Num. AAA8100354

• Gene Names: CD244; 2B4; NAIL; Nmrk; NKR2B4; SLAMF4
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A
• Synonyms: 2B4/CD244; Monoclonal Antibody; Recombinant Anti-2B4/CD244 Antibody; Rabbit Monoclonal; 2B4/CD244 Antibody; Rabbit MAb; CD244 molecule; natural killer cell receptor 2B4; Anti-2B4 Antibody; Anti-NAIL Antibody; Anti-NKR2B4 Antibody; Anti-Nmrk Antibody; Anti-SLAMF4 Antibody; anti-CD244 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 025
• Specificity: Human 2B4/CD244
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CD244 antibody: Western Blot (WB), ELISA (EIA) (Cap), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 1-4uL/mg of lysate; ELISA (Cap): 1:250-1:2000; ELISA (Cap): This antibody will detect Human 2B4/CD244 in ELISA pair set.; In a sandwich ELISA, it can be used as capture antibody when paired with.
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human 2B4/CD244 protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human 2B4/CD244 (rh 2B4/CD244).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-2B4 rabbit monoclonal antibody at 1:500 dilutionLane A: Daudi Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:42 kDaObserved band size:28 kDa(We are unsure as to the identity of these extra bands.))

Immunoprecipitation (IP)

(2B4 was immunoprecipitated using:Lane A:0.5 mg Daudi Whole Cell Lysate2 uL anti-2B4 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-2B4 rabbit monoclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 42 kDaObserved band size: 28 kDa)

Related Product Information for anti-CD244 antibody

The CD244 antigen, also known as 2B4, is a cell surface glycoprotein implicated in the regulation of natural killer and T lymphocyte function. 2B4 is a member of the signaling lymphocyte activation molecule (SLAM)-related receptor family and is important for stimulating NK cell cytotoxicity and cytokine production, which is expressed on all NK cells, a subpopulation of T cells, monocytes and basophils. The 2B4 antigen identified on NK cells and T cells is capable of transmitting stimulatory signals and non-MHC-restricted killing. Reported as an activating receptor, human 2B4 can effectively activate and enhance NK cell-mediated cytotoxicity, induce secretion of IFN-gamma and matrix metalloproteinases (MMPs), as well as NK cell invasiveness. As a cell surface glycoprotein of the Ig-superfamily structurally related to CD2-like molecules such as CD2, CD48, CD58, CD84, Ly-9, and SLAM, 2B4 (CD244) is expressed on all human NK cells, a subpopulation of T cells, basophils and monocytes. 2B4 activates NK cell mediated cytotoxicity, induces secretion of IFN-gamma and matrix metalloproteinases, and NK cell invasiveness.

Product Categories/Family for anti-CD244 antibody

Cancer Drug Targets ITIM/ITAM Immunoreceptors and Related Molecules

References

Nakajima H, et al. (2000) 2B4: an NK cell activating receptor with unique specificity and signal transduction mechanism. Hum Immunol. 61(1): 39-43.Chuang SS, et al. (2001) 2B4 (CD244)-mediated activation of cytotoxicity and IFN-gamma release in human NK cells involves distinct pathways. J Immunol. 167(11): 6210-6.McNerney ME, et al. (2005) 2B4 (CD244) is a non-MHC binding receptor with multiple functions on natural killer cells and CD8+ T cells. Mol Immunol. 42(4): 489-94.Mathew SO, et al. (2009) Functional role of human NK cell receptor 2B4 (CD244) isoforms. Eur J Immunol. 39(6): 1632-41.

NCBI and Uniprot Product Information

• NCBI GI #: 7706529
• NCBI GeneID: 51744
• NCBI Accession #: NP_057466.1
• NCBI GenBank Nucleotide #: NM_016382.3
• Molecular Weight: 30,784 Da
• NCBI Official Full Name: natural killer cell receptor 2B4 isoform 1
• NCBI Official Synonym Full Names: CD244 molecule
• NCBI Official Symbol: CD244
• NCBI Official Synonym Symbols: 2B4; NAIL; Nmrk; NKR2B4; SLAMF4
• NCBI Protein Information: natural killer cell receptor 2B4
• Protein Family: Natural killer cell receptor

NCBI Description

This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]