Typical Testing Data/Standard Curve (for reference only)
(Human CD244/2B4 PicoKine ELISA Kit standard curve )
Human CD244/2B4 ELISA Kit | CD244 elisa kit
Human CD244/2B4 PicoKine ELISA Kit
Gene Names
CD244; 2B4; NAIL; Nmrk; NKR2B4; SLAMF4
Reactivity
Human
Synonyms
CD244/2B4; Human CD244/2B4 PicoKine ELISA Kit; Natural killer cell receptor 2B4; CD244 molecule; natural killer cell receptor 2B4; 2B4; C9.1; CD244; CD244 antigen; CD244 molecule natural killer cell receptor 2B4; CD244 natural killer cell receptor 2B4; CD244_HUMAN; F730046O15Rik; h2B4; Ly90; Natural killer cell activation-inducing ligand; NK cell activation inducing ligand; NK cell activation inducing ligand NAIL; NK cell activation-inducing ligand; NK cell type I receptor protein 2B4; NKR2B4; Nmrk; Non-MHC restricted killing associated; OTTHUMP00000027884; p38; signaling lymphocytic activation molecule 4; SLAM family; member 4; SLAMF4; CD244 elisa kit
Reactivity
Human
Specificity
Natural and recombinant human CD244
Sequence Length
370
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
62.5pg/ml-4000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Human CD244/2B4 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Human CD244/2B4 PicoKine ELISA Kit standard curve )
Related Product Information for CD244 elisa kit
Principle of the assay: human CD244 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD244 has been precoated onto 96-well plates. Standards(NSO, C22-R221) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD244 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD244 amount of sample captured in plate.
Background: CD244 (Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Members of the CD2 family adhere to each other instead,the cell surface glycoprotein 2B4 is related to CD2 and is implicated in the regulation of NK- and T-cell function. The 2B4 gene was mapped to 1q22 on gene map, where SLAM, CD48, CD84 and LY9 are also located. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, It was found that CD48 binds to 2B4. BIAcore surface plasmon resonance analysis showed that the affinity of CD48 for 2B4 is approximately 10-fold higher than that shown for CD48 and CD2.
Background: CD244 (Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Members of the CD2 family adhere to each other instead,the cell surface glycoprotein 2B4 is related to CD2 and is implicated in the regulation of NK- and T-cell function. The 2B4 gene was mapped to 1q22 on gene map, where SLAM, CD48, CD84 and LY9 are also located. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, It was found that CD48 binds to 2B4. BIAcore surface plasmon resonance analysis showed that the affinity of CD48 for 2B4 is approximately 10-fold higher than that shown for CD48 and CD2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
30,784 Da
NCBI Official Full Name
Natural killer cell receptor 2B4
NCBI Official Synonym Full Names
CD244 molecule, natural killer cell receptor 2B4
NCBI Official Symbol
CD244
NCBI Official Synonym Symbols
2B4; NAIL; Nmrk; NKR2B4; SLAMF4
NCBI Protein Information
natural killer cell receptor 2B4; h2B4; SLAM family member 4; NK cell activation-inducing ligand; NK cell type I receptor protein 2B4; NK cell activation inducing ligand NAIL; signaling lymphocytic activation molecule 4
UniProt Protein Name
Natural killer cell receptor 2B4
Protein Family
UniProt Gene Name
CD244
UniProt Synonym Gene Names
2B4; NAIL; NKR2B4; h2B4; SLAMF4
UniProt Entry Name
CD244_HUMAN
NCBI Description
This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]
Uniprot Description
CD244: Modulate other receptor-ligand interactions to enhance leukocyte activation. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, misc.
Chromosomal Location of Human Ortholog: 1q23.3
Cellular Component: plasma membrane; integral to membrane; external side of plasma membrane
Molecular Function: protein binding; receptor activity
Biological Process: blood coagulation; signal transduction; leukocyte migration
Disease: Rheumatoid Arthritis
Research Articles on CD244
Similar Products
Product Notes
The Human CD244 cd244 (Catalog #AAA176534) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA176534 ELISA Kit recognizes Human CD244. It is sometimes possible for the material contained within the vial of "CD244/2B4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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