Testing Data
2B4/CD244 ELISA Pair Set | CD244 elisa pair set
2B4/CD244 Matched ELISA Antibody Pair Set, Human
Synonyms
2B4/CD244; 2B4/CD244 Matched ELISA Antibody Pair Set; Human; Human 2B4/CD244 ELISA Pair Set; CD244 molecule; natural killer cell receptor 2B4; 2B4 Matched ELISA Antibody Pair Set; NAIL Matched ELISA Antibody Pair Set; NKR2B4 Matched ELISA Antibody Pair Set; Nmrk Matched ELISA Antibody Pair Set; SLAMF4 Matched ELISA Antibody Pair Set; CD244 elisa pair set
Specificity
Quantitative determination of Human 2B4/CD244
Assay Type
Solid Phase Sandwich ELISA
Detection Range
125-8000pg/mL
Sensitivity
125pg/ml
Materials Provided
1. Capture Antibody 0.5 mg/mL of rabbit anti-2B4 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in CBS before coating.
2. Detection Antibody 0.25 mg/mL rabbit anti-2B4 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4). Dilute to working concentration of 0.0625ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 56 ng of recombinant 2B4. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 8ng/mL is recommended.
2. Detection Antibody 0.25 mg/mL rabbit anti-2B4 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4). Dilute to working concentration of 0.0625ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 56 ng of recombinant 2B4. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 8ng/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for CD244 elisa pair set
The CD244 antigen, also known as 2B4, is a cell surface glycoprotein implicated in the regulation of natural killer and T lymphocyte function. 2B4 is a member of the signaling lymphocyte activation molecule (SLAM)-related receptor family and is important for stimulating NK cell cytotoxicity and cytokine production, which is expressed on all NK cells, a subpopulation of T cells, monocytes and basophils. The 2B4 antigen identified on NK cells and T cells is capable of transmitting stimulatory signals and non-MHC-restricted killing. Reported as an activating receptor, human 2B4 can effectively activate and enhance NK cell-mediated cytotoxicity, induce secretion of IFN-gamma and matrix metalloproteinases (MMPs), as well as NK cell invasiveness. As a cell surface glycoprotein of the Ig-superfamily structurally related to CD2-like molecules such as CD2, CD48, CD58, CD84, Ly-9, and SLAM, 2B4 (CD244) is expressed on all human NK cells, a subpopulation of T cells, basophils and monocytes. 2B4 activates NK cell mediated cytotoxicity, induces secretion of IFN-gamma and matrix metalloproteinases, and NK cell invasiveness.
This 2B4/CD244 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human 2B4/CD244. It contains Human 2B4/CD244 capture antibody, Human 2B4/CD244 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human 2B4/CD244 protein.
This 2B4/CD244 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human 2B4/CD244. It contains Human 2B4/CD244 capture antibody, Human 2B4/CD244 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human 2B4/CD244 protein.
Product Categories/Family for CD244 elisa pair set
References
Nakajima H, et al. (2000) 2B4: an NK cell activating receptor with unique specificity and signal transduction mechanism. Hum Immunol. 61(1): 39-43.Chuang SS, et al. (2001) 2B4 (CD244)-mediated activation of cytotoxicity and IFN-gamma release in human NK cells involves distinct pathways. J Immunol. 167(11): 6210-6.McNerney ME, et al. (2005) 2B4 (CD244) is a non-MHC binding receptor with multiple functions on natural killer cells and CD8+ T cells. Mol Immunol. 42(4): 489-94.Mathew SO, et al. (2009) Functional role of human NK cell receptor 2B4 (CD244) isoforms. Eur J Immunol. 39(6): 1632-41.
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Product Notes
The CD244 (Catalog #AAA8118175) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "2B4/CD244, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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