2B4/CD244 Active Protein | CD244 active protein

2B4/CD244 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8119958

• Gene Names: CD244; 2B4; NAIL; Nmrk; NKR2B4; SLAMF4
• Purity: >97% as determined by SDS-PAGE
• Synonyms: 2B4/CD244; 2B4/CD244 Protein; Human; Recombinant (hFc Tag); Human 2B4/SLAMF/CD244 Protein (Fc Tag); 2B4 Protein; NAIL Protein; NKR2B4 Protein; Nmrk Protein; SLAMF4 Protein; CD244 molecule; natural killer cell receptor 2B4; CD244 active protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg221

• Species: Human
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD48 at 10ug/ml (100uL/well) can bind recombinant human 2B4/CD244 with a linear range of 0.004-0.4ug/ml
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Cys 22
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Arg221) of human 2B4/CD244 (NP_057466.1) precursor was expressed with the C-terminal fused Fc region of human IgG1.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized human CD48 at 10 ug/ml (100 ul/well) can bind recombinant human 2B4 / CD244 with a linear range of 0.004-0.4 ug/ml.)

Related Product Information for CD244 active protein

Background: The CD244 antigen, also known as 2B4, is a cell surface glycoprotein implicated in the regulation of natural killer and T lymphocyte function. 2B4 is a member of the signaling lymphocyte activation molecule (SLAM)-related receptor family and is important for stimulating NK cell cytotoxicity and cytokine production, which is expressed on all NK cells, a subpopulation of T cells, monocytes and basophils. The 2B4 antigen identified on NK cells and T cells is capable of transmitting stimulatory signals and non-MHC-restricted killing. Reported as an activating receptor, human 2B4 can effectively activate and enhance NK cell-mediated cytotoxicity, induce secretion of IFN-gamma and matrix metalloproteinases (MMPs), as well as NK cell invasiveness. As a cell surface glycoprotein of the Ig-superfamily structurally related to CD2-like molecules such as CD2, CD48, CD58, CD84, Ly-9, and SLAM, 2B4 (CD244) is expressed on all human NK cells, a subpopulation of T cells, basophils and monocytes. 2B4 activates NK cell mediated cytotoxicity, induces secretion of IFN-gamma and matrix metalloproteinases, and NK cell invasiveness.

Product Categories/Family for CD244 active protein

Cancer Drug Targets ITIM/ITAM Immunoreceptors and Related Molecules

References

Nakajima H, et al. (2000) 2B4: an NK cell activating receptor with unique specificity and signal transduction mechanism. Hum Immunol. 61(1): 39-43.Chuang SS, et al. (2001) 2B4 (CD244)-mediated activation of cytotoxicity and IFN-gamma release in human NK cells involves distinct pathways. J Immunol. 167(11): 6210-6.McNerney ME, et al. (2005) 2B4 (CD244) is a non-MHC binding receptor with multiple functions on natural killer cells and CD8+ T cells. Mol Immunol. 42(4): 489-94.Mathew SO, et al. (2009) Functional role of human NK cell receptor 2B4 (CD244) isoforms. Eur J Immunol. 39(6): 1632-41.

NCBI and Uniprot Product Information

• NCBI GI #: 7706529
• NCBI GeneID: 51744
• NCBI Accession #: NP_057466.1
• NCBI GenBank Nucleotide #: NM_016382.3
• Molecular Weight: 30,784 Da
• NCBI Official Full Name: natural killer cell receptor 2B4 isoform 1
• NCBI Official Synonym Full Names: CD244 molecule
• NCBI Official Symbol: CD244
• NCBI Official Synonym Symbols: 2B4; NAIL; Nmrk; NKR2B4; SLAMF4
• NCBI Protein Information: natural killer cell receptor 2B4
• Protein Family: Natural killer cell receptor

NCBI Description

This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]