Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Nuclease-sensitive element-binding protein 1 (YBX1) Standard Curve.)
Human Nuclease-Sensitive Element-Binding Protein 1 (YBX1) ELISA Kit | YBX1 elisa kit
Human Nuclease-Sensitive Element-Binding Protein 1 (YBX1) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Nuclease-sensitive element-binding protein 1 (YBX1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Nuclease-sensitive element-binding protein 1 (YBX1) Standard Curve.)
Background: Y box binding protein 1is a human protein. Current research is examining its involvement in cancer, and particularly in the metastasis of cancerous cells or prevention thereof. The deduced 322-amino acid protein contains 4 putative DNA-binding domains, 3 of which are rich in basic amino acids. Two of these basic regions, basic-1 and basic-2, form the double-strand DNA-binding domain of the protein. NSEP1 also has a pro/ser/thr-rich domain and an asp/glu/gln-rich domain, both of which are reminiscent of activation domains. It also contains an octapeptide single-strand DNA-binding motif that shares weak homology with the ribonucleoprotein consensus sequence of RNA-binding proteins.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a highly conserved cold shock domain protein that has broad nucleic acid binding properties. The encoded protein functions as both a DNA and RNA binding protein and has been implicated in numerous cellular processes including regulation of transcription and translation, pre-mRNA splicing, DNA reparation and mRNA packaging. This protein is also a component of messenger ribonucleoprotein (mRNP) complexes and may have a role in microRNA processing. This protein can be secreted through non-classical pathways and functions as an extracellular mitogen. Aberrant expression of the gene is associated with cancer proliferation in numerous tissues. This gene may be a prognostic marker for poor outcome and drug resistance in certain cancers. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on multiple chromosomes. [provided by RefSeq, Sep 2015]
Uniprot Description
YB-1: a nuclear protein that binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors CCAAT-containing Y-box of HLA class II genes. Component of cytoplasmic messenger ribonucleoprotein particles (mRNPs). Interacts with AKT1, SFRS9, THOC4, MSH2, XRCC5, WRN and NCL. Can bind to DNA as a homomeric form, (EFI-A)n or as a heteromeric form in association with EFI-B. Homodimer in the presence of ATP. Involved in cisplatin resistance. Seems to be a negative regulatory factor. May play a role in both transcriptional and translational regulation of spermatogenesis. Found at very low levels at day 10 and levels increase at day 15 and persist throughout adulthood.
Protein type: Transcription factor; RNA-binding; RNA splicing; RNA processing
Chromosomal Location of Human Ortholog: 1p34
Cellular Component: nucleoplasm; nuclear membrane; intracellular membrane-bound organelle; stress granule; cytoplasm; nucleus; ribonucleoprotein complex; U12-dependent spliceosome
Molecular Function: protein binding; DNA binding; RNA binding; double-stranded DNA binding; transcription factor activity; single-stranded DNA binding
Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; negative regulation of striated muscle cell differentiation; regulation of transcription, DNA-dependent; in utero embryonic development; positive regulation of cell division; RNA splicing; positive regulation of transcription from RNA polymerase II promoter; gene expression; negative regulation of transcription from RNA polymerase II promoter
Research Articles on YBX1
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Product Notes
The Human YBX1 ybx1 (Catalog #AAA9713678) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713678 ELISA Kit recognizes Human YBX1. It is sometimes possible for the material contained within the vial of "Nuclease-Sensitive Element-Binding Protein 1 (YBX1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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