Rat 72 kDa type IV collagenase ELISA Kit | Mmp2 elisa kit

Rat 72 kDa type IV collagenase ELISA Kit

Cat. Num. AAA2884273

• Reactivity: Rat
• Synonyms: 72 kDa type IV collagenase; Rat 72 kDa type IV collagenase ELISA Kit; 72 kDa gelatinase; Gelatinase A; Matrix metalloproteinase-2; MMP-2; Mmp2; 3.4.24.24; Mmp2 elisa kit

• Reactivity: Rat
• Specificity: This assay recognizes recombinant and natural rat 72 kDa type IV collagenase. No significant cross-reactivity or interference was observed.
• Sequence Length: 662

• Assay Type: Sandwich
• Detection Range: 0.312-20 ng/mL
• Sensitivity: 0.168 ng/mL
• Intra-Assay CV: <=3.2%
• Inter-Assay CV: <=7.8%
• Recovery: 97%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Mmp2 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of rat 72 kDa type IV collagenase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to rat 72 kDa type IV collagenase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for rat 72 kDa type IV collagenase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain rat 72 kDa type IV collagenase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of rat 72 kDa type IV collagenase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 146262019
• NCBI GeneID: 81686
• NCBI Accession #: NP_112316.2
• NCBI GenBank Nucleotide #: NM_031054.2
• UniProt Accession #: P33436; P97581; Q6GMM9
• Molecular Weight: 74,149 Da
• NCBI Official Full Name: 72 kDa type IV collagenase
• NCBI Official Synonym Full Names: matrix metallopeptidase 2
• NCBI Official Symbol: Mmp2
• NCBI Protein Information: 72 kDa type IV collagenase
• UniProt Protein Name: 72 kDa type IV collagenase
• Protein Family: 72 kDa type IV collagenase
• UniProt Gene Name: Mmp2
• UniProt Synonym Gene Names: MMP-2
• UniProt Entry Name: MMP2_RAT

NCBI Description

plays important roles in wound healing, angiogenesis, platelet aggregation, and tumor metastasis; mediates the response of the intrinsic glomerular mesangial cell to inflammatory stimuli [RGD, Feb 2006]

Uniprot Description

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues ().