Typical Testing Data/Standard Curve (for reference only)
Mouse Kynurenine 3-monooxygenase ELISA Kit | Kmo elisa kit
Mouse Kynurenine 3-monooxygenase ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kynurenine 3-monooxygenase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Kynurenine 3-monooxygenase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Kynurenine 3-monooxygenase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Kynurenine 3-monooxygenase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
KMO: Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid, a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Quinolinic acid may also affect NMDA receptor signaling in pancreatic beta cells, osteoblasts, myocardial cells, and the gastrointestinal tract. Belongs to the aromatic-ring hydroxylase family. KMO subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Amino Acid Metabolism - tryptophan; EC 1.14.13.9; Membrane protein, multi-pass; Membrane protein, integral; Mitochondrial; Oxidoreductase
Cellular Component: integral to membrane; membrane; mitochondrial inner membrane; mitochondrial outer membrane; mitochondrion
Molecular Function: FAD binding; kynurenine 3-monooxygenase activity; monooxygenase activity; NAD(P)H oxidase activity; oxidoreductase activity
Biological Process: NAD metabolic process; pyridine nucleotide biosynthetic process; quinolinate biosynthetic process; response to salt stress; tryptophan catabolic process; tryptophan catabolic process to kynurenine
Research Articles on Kmo
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Product Notes
The Mouse Kmo kmo (Catalog #AAA2882388) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2882388 ELISA Kit recognizes Mouse Kmo. It is sometimes possible for the material contained within the vial of "Kynurenine 3-monooxygenase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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