Rat Cystathionine gamma-lyase ELISA Kit | Cth elisa kit

Rat Cystathionine gamma-lyase ELISA Kit

Cat. Num. AAA288180

• Gene Names: Cth; H
• Reactivity: Rat
• Synonyms: Cystathionine gamma-lyase; Rat Cystathionine gamma-lyase ELISA Kit; Cth elisa kit

• Reactivity: Rat
• Specificity: This assay recognizes recombinant and natural rat Cystathionine gamma-lyase. No significant cross-reactivity or interference was observed.
• Sequence Length: 423

• Assay Type: Sandwich
• Detection Range: 0.78-50 ng/ml
• Sensitivity: 0.399 ng/mL
• Intra-Assay CV: <=5.0%
• Inter-Assay CV: <=6.3%
• Recovery: 96%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Cth elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cystathionine gamma-lyase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cystathionine gamma-lyase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cystathionine gamma-lyase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Cystathionine gamma-lyase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 8393215
• NCBI GeneID: 24962
• NCBI Accession #: NP_058770.1
• NCBI GenBank Nucleotide #: NM_017074.1
• UniProt Accession #: P18757
• Molecular Weight: 43,605 Da
• NCBI Official Full Name: cystathionine gamma-lyase
• NCBI Official Synonym Full Names: cystathionine gamma-lyase
• NCBI Official Symbol: Cth
• NCBI Official Synonym Symbols: H
• NCBI Protein Information: cystathionine gamma-lyase
• UniProt Protein Name: Cystathionine gamma-lyase
• Protein Family: Cystathionine gamma-lyase
• UniProt Gene Name: Cth
• UniProt Synonym Gene Names: PRB-RA
• UniProt Entry Name: CGL_RAT

NCBI Description

putative cystathionine gamma-lyase enzyme [RGD, Feb 2006]

Uniprot Description

CTH: Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function. Defects in CTH are the cause of cystathioninuria (CSTNU). It is an autosomal recessive phenotype characterized by abnormal accumulation of plasma cystathionine, leading to increased urinary excretion. Belongs to the trans-sulfuration enzymes family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lyase; Amino Acid Metabolism - glycine, serine and threonine; Energy Metabolism - nitrogen; Amino Acid Metabolism - cysteine and methionine; Other Amino Acids Metabolism - selenoamino acid; Cell cycle regulation; EC 4.4.1.1

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: calmodulin binding; catalytic activity; cystathionine beta-lyase activity; cystathionine gamma-lyase activity; pyridoxal phosphate binding

Biological Process: activation of NF-kappaB transcription factor; cysteine biosynthetic process; cysteine biosynthetic process via cystathionine; glutathione metabolic process; homocysteine metabolic process; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein homotetramerization; protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine; transsulfuration