Rabbit matrix metallopeptidase 3 (stromelysin 1, progelatinase) ELISA Kit | MMP3 elisa kit

Rabbit matrix metalloproteinase 3, MMP-3 ELISA Kit

Cat. Num. AAA700078

• Reactivity: Rabbit
• Synonyms: matrix metallopeptidase 3 (stromelysin 1; progelatinase); Rabbit matrix metalloproteinase 3; MMP-3 ELISA Kit; MMP-3 ELISA kit; CHDS6; MGC126102; MGC126103; MGC126104; MMP-3; SL-1; STMY; STMY1; STR1; matrix metalloproteinase 3; matrix metalloproteinase 3 (stromelysin 1; progelatinase) ; proteoglycanase; transin-1; MMP3 elisa kit

• Reactivity: Rabbit
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rabbit MMP3. No significant cross-reactivity or interference between Rabbit MMP3 and analogues was observed.
• Sequence Length: 478

• Samples: Serum, plasma and tissue homogenates
• Detection Range: 0.156 ng/ml-10 ng/ml
• Sensitivity: 0.039 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Developmental Protein
• Protein Biological Process 3: Collagen degradation
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP3 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP-3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for MMP-3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain MMP-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MMP-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 126723161
• NCBI GeneID: 100009111
• NCBI Accession #: NP_001075749.1
• NCBI GenBank Nucleotide #: NM_001082280.1
• UniProt Accession #: P28863
• Molecular Weight: 53,942 Da
• NCBI Official Full Name: stromelysin-1 preproprotein
• NCBI Official Symbol: MMP3
• NCBI Protein Information: stromelysin-1; SL-1; MMP-3; transin-1; matrix metalloproteinase 3; matrix metalloproteinase-3
• UniProt Protein Name: Stromelysin-1
• Protein Family: Stromelysin
• UniProt Gene Name: MMP3
• UniProt Synonym Gene Names: SL-1; MMP-3
• UniProt Entry Name: MMP3_RABIT

Uniprot Description

Function: Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

Catalytic activity: Preferential cleavage where P1', P2' and P3' are hydrophobic residues.

Cofactor: Binds 4 calcium ions per subunit