METTL16 recombinant protein

Recombinant METTL16 protein

Cat. Num. AAA389142

• Gene Names: METTL16; METT10D
• Synonyms: METTL16; Recombinant METTL16 protein; Methyltransferase Like 16; N6-Adenosine-Methyltransferase METTL16; METT10D; METTL16 recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant METTL16 protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant METTL16 protein gel 10% SDS-PAGE Coomassie staining MW: 64.9 kDa Purity: >95% )

Related Product Information for METTL16 recombinant protein

Short Description: Recombinant human METTL16 protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_076991.3) with an N-terminal DYKDDDDK tag. The molecular weight of the protein is 64.9 kDa.

Background: METTL16 (Methyltransferase-like protein 16), also known as U6 small nuclear RNA (adenine-(43)-N(6))- methyltransferase or METT10D (Methyltransferase 10 Domain-Containing Protein), is a RNA N6-methyltransferase that methylates adenosine residues of a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs). In contrast to the METTL3-METTL14 heterodimer, METTL16 is only able to methylate a limited number of RNAs: requires both a 5UACAGAGAA-3 nonamer sequence and a specific RNA structure. It also plays a key role in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts. In presence of S-adenosyl-L-methionine, METTL16 binds the 3-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, leading to intron retention and preventing MAT2A mRNA splicing. While in S-adenosyl-L-methionine-limiting conditions, it binds the 3-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, leading to stimulate splicing of the MAT2A retained intron, and promoting expression of MAT2A.

Product Categories/Family for METTL16 recombinant protein

Recombinant Proteins; Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 122114654
• NCBI GeneID: 79066
• NCBI Accession #: NP_076991.3
• NCBI GenBank Nucleotide #: NM_024086.3
• UniProt Accession #: Q86W50; Q86TE5; Q96T16; Q9BVG7; D3DTI8
• Molecular Weight: 63,621 Da
• NCBI Official Full Name: methyltransferase-like protein 16
• NCBI Official Synonym Full Names: methyltransferase like 16
• NCBI Official Symbol: METTL16
• NCBI Official Synonym Symbols: METT10D
• NCBI Protein Information: methyltransferase-like protein 16; putative methyltransferase METT10D; methyltransferase 10 domain containing; methyltransferase 10 domain-containing protein
• UniProt Protein Name: Methyltransferase-like protein 16
• Protein Family: Methyltransferase-like protein
• UniProt Gene Name: METTL16
• UniProt Synonym Gene Names: METT10D
• UniProt Entry Name: MET16_HUMAN

Uniprot Description

MGC3329: Probable methyltransferase. Belongs to the methyltransferase superfamily. METTL16/RlmF family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; EC 2.1.1.-

Chromosomal Location of Human Ortholog: 17p13.3

Molecular Function: methyltransferase activity

Biological Process: methylation