METTL14 recombinant protein

Recombinant METTL14 protein

Cat. Num. AAA388271

• Gene Names: METTL14; hMETTL14
• Synonyms: METTL14; Recombinant METTL14 protein; Methyltransferase Like 14; EC 2.1.1.62; N6-methyltransferase; METTL14 recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant METTL14 protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant METTL14 protein gel Recombinant METTL14 was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 53.3 kDa Purity: > 79%)

Related Product Information for METTL14 recombinant protein

Short Description: Recombinant METTL14 protein was expressed in a Baculovirus expression system as the full-length protein (accession number NP_066012.1) with an N-terminal DYKDDDDK-Tag. The molecular weight of METTL14 is 53.3 kDa. It is suitable for analysis of RNA methylation and modification, RNA splicing, processing and stability studies.

Background: N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messager RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also takes place in other RNA molecules, such as primary miRNA (pri-miRNAs). METTL14 (methyltransferase-like 14) forms a stable N6-methyltransferase heterodimer complex with METTL3, which catalyzes the generation of m6A modification on mammalian nuclear RNAs. METTL3 is the catalytically active subunit, while METTL14 plays a structural role critical for substrate recognition.

Product Categories/Family for METTL14 recombinant protein

Recombinant Proteins; Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 24308265
• NCBI GeneID: 57721
• NCBI Accession #: NP_066012.1
• NCBI GenBank Nucleotide #: NM_020961.3
• UniProt Accession #: Q9HCE5; Q969V2; A6NIG1
• Molecular Weight: 52,150 Da
• NCBI Official Full Name: N6-adenosine-methyltransferase non-catalytic subunit
• NCBI Official Synonym Full Names: methyltransferase like 14
• NCBI Official Symbol: METTL14
• NCBI Official Synonym Symbols: hMETTL14
• NCBI Protein Information: N6-adenosine-methyltransferase non-catalytic subunit; N6-adenosine-methyltransferase subunit METTL14
• UniProt Protein Name: N6-adenosine-methyltransferase non-catalytic subunit
• Protein Family: N6-adenosine-methyltransferase
• UniProt Gene Name: METTL14
• UniProt Synonym Gene Names: hMETTL14

Uniprot Description

The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N6 position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis (PubMed:24316715, PubMed:24407421, PubMed:25719671, PubMed:27373337, PubMed:27281194). In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core (PubMed:27627798, PubMed:27373337, PubMed:27281194). N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (). In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (). M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (). M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells ().